College of Chemistry and Materials Science, Shaanxi Normal University, 199 South Chang'an Road, Xi'an 710062, PR China.
Talanta. 2010 Apr 15;81(1-2):477-81. doi: 10.1016/j.talanta.2009.12.028. Epub 2009 Dec 23.
A novel and high selectivity flow-injection chemiluminescence (FI-CL) system with diperiodatoargentate(III) (DPA) is developed for the determination of uric acid for the first time. It is based on the reaction of diperiodatoargentate(III) (DPA) with uric acid in alkaline medium to emit CL. With the peak height as a quantitative parameter applying optimum working conditions, the relative CL intensity was linear with the uric acid concentration in the range of 4.0 x 10(-7)-2.0 x 10(-4) mol L(-1) with a detection limit of 1.2 x 10(-7) mol L(-1) (3 sigma). The relative standard deviation (RSD) was 2.1% for 5.0 x 10(-5) mol L(-1) uric acid (n=7). The proposed method held higher selectivity than other CL methods and was applied to determination of uric acid in human serum. The possible CL reaction mechanism was also discussed briefly.
一种新颖的、高选择性的流动注射化学发光（FI-CL）体系，以高氯酸银（Ag(IO3)2）为氧化剂，用于尿酸的测定。在碱性介质中，Ag(IO3)2 与尿酸反应产生 CL。在最佳实验条件下，以峰高为定量参数，CL 强度与尿酸浓度在 4.0×10^-7-2.0×10^-4mol/L 范围内呈线性关系，检出限为 1.2×10^-7mol/L（3σ）。对 5.0×10^-5mol/L 的尿酸进行 7 次平行测定，相对标准偏差（RSD）为 2.1%。与其他 CL 方法相比，该方法具有更高的选择性，并已成功应用于人血清中尿酸的测定。简要讨论了可能的 CL 反应机制。
