Key Laboratory of Synthetic and Natural Functional Molecule, Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710069, China.
J Anal Methods Chem. 2013;2013:854041. doi: 10.1155/2013/854041. Epub 2013 Oct 22.
Based on the inhibitory effect of uric acid (UA) on luminol-Co(2+) chemiluminescence (CL) system, a sensitive method for the determination of UA at nanomolar level by flow injection (FI) CL was proposed. The proposed method was successfully applied to real-time monitoring of UA excretion in human 24 h urine with different food intake, showing that meats, vegetables, and porridge intake caused differential UA excretions of 879, 798, and 742 mg, respectively. It was also found that UA concentrations in urine under the three kinds of food intake simultaneously reached maximum at 2 h after meals with the values of 417, 318, and 288 μg mL(-1), respectively. The UA concentration in human serum was also determined by this approach, and the possible mechanism of luminol-Co(2+)-UA CL reaction was discussed in detail.
基于尿酸（UA）对鲁米诺-钴（2+）化学发光（CL）体系的抑制作用，提出了一种通过流动注射（FI）CL 测定纳摩尔级 UA 的灵敏方法。该方法成功应用于不同食物摄入的人 24 小时尿液中 UA 排泄的实时监测，结果表明，肉类、蔬菜和粥的摄入分别导致 UA 的排泄量为 879、798 和 742mg。还发现，三种食物摄入同时在餐后 2 小时达到 UA 浓度的峰值，其值分别为 417、318 和 288μg·mL（-1）。还通过该方法测定了人血清中的 UA 浓度，并详细讨论了鲁米诺-钴（2+）-UA CL 反应的可能机制。
