顶空溶剂微萃取-气相色谱-质谱联用分析小茴香中的挥发性化合物
Headspace solvent microextraction-gas chromatography-mass spectrometry for the analysis of volatile compounds from Foeniculum vulgare Mill.
作者信息
Fang Lianghua, Qi Meiling, Li Ting, Shao Qinglong, Fu Ruonong
机构信息
Department of Chemistry, School of Science, Beijing Institute of Technology, Beijing 100081, China.
出版信息
J Pharm Biomed Anal. 2006 Jun 7;41(3):791-7. doi: 10.1016/j.jpba.2006.01.024. Epub 2006 Feb 20.
A novel and rapid headspace solvent microextraction followed by gas chromatography-mass spectrometry (HSME-GC-MS) for the analysis of the volatile compounds of Foeniculum vulgare Mill is described. HSME parameters including extracting solvent, extraction temperature and time, headspace volume and particle size were optimized. As a result, benzyl alcohol was finally used for the extraction at 70 degrees C for 20 min with headspace volume of 12.1 ml and particle size of 120 mesh. Under the determined conditions, the powered samples of Foeniculum vulgare Mill were directly applied for the analysis. A comparison of HSME-GC-MS, solid phase microextraction (SPME)-GC-MS and steam distillation (SD)-GC-MS methods was made and showed that the HSME-GC-MS method was simple, inexpensive and effective and can be used for the analysis of volatile compounds in traditional Chinese medicines (TCMs).
本文描述了一种新颖、快速的顶空溶剂微萃取结合气相色谱 - 质谱联用（HSME - GC - MS）方法，用于分析小茴香（Foeniculum vulgare Mill）中的挥发性化合物。对HSME参数进行了优化，包括萃取溶剂、萃取温度和时间、顶空体积以及粒径。结果表明，最终选用苯甲醇在70℃下萃取20分钟，顶空体积为12.1 ml，粒径为120目。在确定的条件下，直接对小茴香粉末样品进行分析。对HSME - GC - MS、固相微萃取（SPME）- GC - MS和水蒸气蒸馏（SD）- GC - MS方法进行了比较，结果表明HSME - GC - MS方法简单、廉价且有效，可用于分析中药中的挥发性化合物。
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