Department of Chemistry, McGill University, 801 Sherbrooke St W., Montreal, Qc, Canada.
Talanta. 2010 Apr 15;81(1-2):722-6. doi: 10.1016/j.talanta.2009.12.001. Epub 2009 Dec 16.
Great variations in pollutant concentrations are observed in the environment and pre-concentration is often required to detect trace contaminants in water samples. This paper presents a novel solid phase-extraction device integrated onto a centrifugal microfluidic platform for rapid on-site pre-concentration and screening of organic contaminants in aqueous samples. In-column fluorescence and absorbance measurements are obtained directly from an analyte trapped on the top of a solid phase extraction microcolumn. Results are presented for the representative fluorophore fluorescein and the polycyclic aromatic hydrocarbon anthracene. An absolute detection limit of 20 ng was obtained for anthracene using a simple light emitting diode for fluorescence excitation. One of the main advantages of this device is that only a simple motor is needed to induce liquid flow, making simultaneous on-site extraction and measurement of multiple samples easy while minimizing sample losses and contamination.
在环境中观察到污染物浓度的巨大变化,因此通常需要进行预浓缩以检测水样中的痕量污染物。本文提出了一种新型的固相萃取装置,该装置集成在离心微流控平台上,用于快速现场预浓缩和筛选水样中的有机污染物。从固相萃取微柱顶部捕获的分析物直接进行柱内荧光和吸光度测量。结果表明,代表性荧光团荧光素和多环芳烃蒽。使用简单的发光二极管进行荧光激发,可获得蒽的绝对检测限为 20ng。该装置的主要优点之一是,仅需简单的电机即可诱导液体流动,从而便于同时进行现场提取和测量多个样品,同时最大程度地减少样品损失和污染。