Center for Research in Natural Resources, University of the Azores, Apt 1422, 9501-855 Ponta Delgada, Portugal.
Res Vet Sci. 2011 Jun;90(3):526-9. doi: 10.1016/j.rvsc.2010.02.001. Epub 2010 Feb 26.
Urinary bladder tumours in cattle are caused by chronic ingestion of bracken fern and BPV-1/2 infection. The objective of the present study was to assess if BPV-2 was present in urinary bladder lesions from cattle with chronic enzootic haematuria (CEH) from the Azores archipelago (Portugal), in order to gain further information regarding the epidemiologic distribution of this virus. Samples were analysed using PCR specific primers for BPV-2 DNA and an immunohistochemistry for BPV E5 oncoprotein detection. We found a 28% incidence rate of BPV-2 DNA in different types of tumours and cystitis cases (13 out of 46 samples). Tested positive samples for PCR were also positive for the viral E5 oncoprotein; protein immunolabeling was mainly detected within the cytoplasm of urothelial cells, displaying a juxtanuclear distribution. This is the first report of BPV-2 detection in urinary bladder tumours associated with CEH in cattle from the Azores archipelago.
牛的膀胱肿瘤是由慢性摄入蕨类植物和 BPV-1/2 感染引起的。本研究的目的是评估 BPV-2 是否存在于来自亚速尔群岛（葡萄牙）的慢性地方性血尿（CEH）牛的膀胱病变中，以获得有关该病毒流行病学分布的更多信息。使用针对 BPV-2 DNA 的 PCR 特异性引物和针对 BPV E5 致癌蛋白检测的免疫组织化学对样本进行分析。我们在不同类型的肿瘤和膀胱炎病例中发现了 28%的 BPV-2 DNA 发生率（46 个样本中的 13 个）。PCR 检测为阳性的样本也对病毒 E5 致癌蛋白呈阳性；蛋白免疫标记主要在尿路上皮细胞的细胞质中检测到，呈核周分布。这是首次在亚速尔群岛牛的与 CEH 相关的膀胱肿瘤中检测到 BPV-2。
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