Department of Veterinary Pathology & Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Vet Microbiol. 2010 Aug 26;144(3-4):502-4. doi: 10.1016/j.vetmic.2010.02.004. Epub 2010 Feb 10.
Swabs from the brain, eyes and kidneys of tilapia from 11 farms were collected for a period of 2 years. They were grown on blood agar before cultures of suspected Staphylococcus aureus were subjected to ABI STAPH Detection Kit and PCR for identification. They were then grown on oxacillin resistance screening agar base (ORSAB) and subjected to PCR using the MRSA 17 kb forward and reverse primers to identify the methicillin-resistant S. aureus (MRSA). A total of 559 isolates of Staphylococcus spp. were obtained, from which 198 (35%) isolates were identified as S. aureus. Of the 198 S. aureus isolated from tilapias, 98 (50%) were identified as methicillin-resistant S. aureus (MRSA). Since global spread of multi-drug-resistant bacteria has increased in the past decade, this new finding in fish should be of concern.
在为期两年的时间里，从 11 个农场的罗非鱼的大脑、眼睛和肾脏中采集了拭子。它们在血琼脂上生长，然后对疑似金黄色葡萄球菌的培养物进行 ABI STAPH 检测试剂盒和 PCR 鉴定。然后，它们在苯唑西林耐药筛选琼脂基础（ORSAB）上生长，并使用 MRSA 17 kb 正向和反向引物进行 PCR，以鉴定耐甲氧西林金黄色葡萄球菌（MRSA）。共获得 559 株葡萄球菌属分离株，其中 198 株（35%）分离株鉴定为金黄色葡萄球菌。从罗非鱼中分离出的 198 株金黄色葡萄球菌中，有 98 株（50%）鉴定为耐甲氧西林金黄色葡萄球菌（MRSA）。由于过去十年中，全球多药耐药菌的传播有所增加，这种在鱼类中的新发现应该引起关注。
