Elsayed Naeim Doha, Elsayed Eldesoukey Ibrahim, Ahmed Moawad Amgad, Mohammed Ahmed Ashraf
Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh, Egypt.
Vet Res Forum. 2023;14(5):243-248. doi: 10.30466/vrf.2022.551346.3434. Epub 2023 May 15.
Methicillin-resistant (MRSA) infection is a major public health problem. Therefore, this study was aimed to estimate the prevalence of MRSA in various food products. A total number of 204 food samples including raw milk (n = 30), cheese (n = 60), chicken (n = 25), beef (n = 24) and fish (n = 65) were collected from August to November of 2021 within different localities in Kafr El-Sheikh governorate, the northern region of Egypt. All samples were assessed through a series of bacteriological and biochemical techniques to identify MRSA. Out of 204 samples, 52(25.49%) isolates were presumptively identified as MRSA on oxacillin resistance screening agar base media. Of these 52 isolates, 17(32.69%) were characterized as coagulase-positive. For the molecular confirmation of MRSA, all isolates were subjected to polymerase chain reaction assays to detect and . In addition, was identified in all the isolates (100%), whereas, none was positive for . Therefore, based on the detection of , the overall occurrence rate of MRSA among the samples was 8.33%. The isolates were also subjected to antimicrobial susceptibility tests. Cefoxitin, cefuroxime, oxacillin and amoxicillin-clavulanic acid were completely resistant (100%) to the isolates, however, susceptible to vancomycin and ciprofloxacin. Raw milk had the highest prevalence of MRSA (13.30%), followed by chicken (12.00%), fish (9.20%), cheese (5.00%) and beef (4.20%). Due to the possibility of transmission of these strains to humans, the high prevalence of MRSA in various foodstuffs in Egypt poses a potential public health risk.
耐甲氧西林金黄色葡萄球菌（MRSA）感染是一个重大的公共卫生问题。因此，本研究旨在估计各种食品中MRSA的流行率。2021年8月至11月期间，从埃及北部地区卡夫尔谢赫省的不同地点收集了总共204份食品样本，包括生牛奶（n = 30）、奶酪（n = 60）、鸡肉（n = 25）、牛肉（n = 24）和鱼（n = 65）。所有样本均通过一系列细菌学和生化技术进行评估，以鉴定MRSA。在204份样本中，有52份（25.49%）分离株在苯唑西林抗性筛选琼脂基础培养基上被初步鉴定为MRSA。在这52份分离株中，有17份（32.69%）被鉴定为凝固酶阳性。为了对MRSA进行分子确认，所有分离株都进行了聚合酶链反应检测以检测和。此外，所有分离株（100%）中均检测到，而无一例为阳性。因此，基于的检测，样本中MRSA的总体发生率为8.33%。这些分离株还进行了药敏试验。头孢西丁、头孢呋辛、苯唑西林和阿莫西林-克拉维酸对分离株完全耐药（100%），但对万古霉素和环丙沙星敏感。生牛奶中MRSA的流行率最高（13.30%），其次是鸡肉（12.00%）、鱼（9.20%）、奶酪（5.00%）和牛肉（4.20%）。由于这些菌株有可能传播给人类，埃及各种食品中MRSA的高流行率构成了潜在的公共卫生风险。
