Institute for Pharmaceutical Sciences, Department of Pharmaceutical Biology and Biotechnology, Albert-Ludwig-University Freiburg, Stefan-Meier-Strasse 19, 79104 Freiburg, Germany.
J Pharm Biomed Anal. 2010 Oct 10;53(2):137-44. doi: 10.1016/j.jpba.2010.02.001. Epub 2010 Feb 7.
An HPLC method, which allows reliable quantitation of flavonols and other phenolics in birch leaf extracts, was developed and validated. The method was applied to study the bioconversion of flavonols in fermented aqueous extracts. Almost 100% of the flavonols were converted during the 12 months observation period. The generated phenolics as well as consecutive conversion products were identified by HPLC-DAD, LC-MS and GC-MS techniques.
建立并验证了一种高效液相色谱法，可用于可靠定量桦树叶提取物中的类黄酮和其他酚类物质。该方法应用于研究发酵水溶液中类黄酮的生物转化。在 12 个月的观察期内，几乎 100%的类黄酮被转化。通过高效液相色谱-二极管阵列检测、液相色谱-质谱联用和气相色谱-质谱联用技术鉴定了生成的酚类物质及其连续转化产物。
