Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.
J Antimicrob Chemother. 2010 May;65(5):917-23. doi: 10.1093/jac/dkq042. Epub 2010 Feb 26.
Maggots of the blowfly Lucilia sericata are used for the treatment of chronic wounds. Previously we reported that maggot excretions/secretions (ES) break down Staphylococcus aureus biofilms but do not kill the bacteria. As many antibiotics are not effective against biofilms we assessed the effect of combinations of ES and antibiotics on S. aureus biofilms and on the survival of the bacteria released from the biofilms.
Effects of ES, antibiotics (vancomycin, daptomycin or clindamycin) and combinations thereof on S. aureus ATCC 29 213 biofilms and bacterial viability were determined using microtitre plates and in vitro killing assays.
Vancomycin and daptomycin dose-dependently enhanced biofilm formation, whereas clindamycin reduced S. aureus biofilm size. Adding ES to antibiotic incubations caused a complete biofilm breakdown. After a lag time the bacteria derived from biofilms became susceptible to vancomycin and clindamycin, provided that the medium was refreshed. Daptomycin dose-dependently eliminated the biofilm-derived bacteria immediately. Furthermore, it was significantly more effective against bacteria derived from ES-exposed biofilms than those from control biofilms. ES did not affect the activity of the antibiotics against log-phase S. aureus.
Combinations of maggot ES and antibiotics eliminate S. aureus biofilms and the bacteria derived therefrom.
丝光绿蝇（Lucilia sericata）的蛆虫被用于治疗慢性伤口。我们之前报道过蛆虫的分泌物可以分解金黄色葡萄球菌生物膜，但不能杀死细菌。由于许多抗生素对生物膜无效，我们评估了 ES 与抗生素联合使用对金黄色葡萄球菌生物膜以及从生物膜中释放的细菌存活的影响。
使用微量滴定板和体外杀伤试验，测定 ES、抗生素（万古霉素、达托霉素或克林霉素）及其组合对金黄色葡萄球菌 ATCC 29213 生物膜和细菌活力的影响。
万古霉素和达托霉素剂量依赖性地增强生物膜形成，而克林霉素则减少金黄色葡萄球菌生物膜的大小。将 ES 添加到抗生素孵育中会导致完全破坏生物膜。在潜伏期后，从生物膜中分离出来的细菌对万古霉素和克林霉素变得敏感，只要培养基被更新。达托霉素可立即消除生物膜衍生的细菌，并且对 ES 暴露的生物膜衍生的细菌比对照生物膜衍生的细菌更有效。ES 不影响抗生素对对数期金黄色葡萄球菌的活性。
蛆虫 ES 与抗生素的组合可消除金黄色葡萄球菌生物膜及其衍生的细菌。
