Department of Pharmacology, University of Zagreb Medical School, Zagreb, Croatia.
Med Sci Monit. 2010 Mar;16(3):BR81-88.
The effect of systemic and local peptide treatment effective in muscle contusion and then on counteraction of corticosteroid-induced impairment was tested. The pentadecapeptide BPC 157, given without a carrier, improved the healing of transected quadriceps muscle. It also improved muscle healing in rats with muscle crush injury when applied systemically or locally. Importantly, it counteracted corticosteroid-impairment in tendon to bone healing. Thus BPC 157 is proposed as an effective treatment that can improve muscle healing in spite of corticosteroid treatment.
MATERIAL/METHODS: After the gastrocnemius muscle complex had been injured, rats received BPC 157 (intraperitoneally or locally as a cream) and/or 6alpha-methylprednisolone (intraperitoneally) only once (immediately after injury, sacrifice at 2 h) or once daily (final dose 24 hours before sacrifice and/or assessment procedure at days 1, 2, 4, 7, and 14). Muscle healing was evaluated functionally, macroscopically, and histologically.
Without therapy, crushed gastrocnemius muscle complex controls showed limited improvement. 6alpha-methylprednisolone markedly aggravated healing. In contrast, BPC 157 induced faster muscle healing and full function restoration and improved muscle healing despite systemic corticosteroid treatment when given intraperitoneally or locally and demonstrated functionally, macroscopically, and histologically at all investigated intervals.
BPC 157 completely reversed systemic corticosteroid-impaired muscle healing.
本研究旨在测试全身和局部肽类治疗对肌肉挫伤的疗效，以及对皮质类固醇引起的损伤的拮抗作用。非载体肽 BPC 157 可改善横断股四头肌的愈合。当全身或局部应用于肌肉挤压伤大鼠时，它也能改善肌肉愈合。重要的是，它拮抗了皮质类固醇对肌腱-骨愈合的损伤。因此，BPC 157 被提议作为一种有效的治疗方法，即使在皮质类固醇治疗的情况下，也能改善肌肉愈合。
材料/方法：在腓肠肌复合体受伤后，大鼠接受 BPC 157（腹腔内或局部作为乳膏）和/或 6α-甲基强的松龙（腹腔内），仅一次（受伤后立即，2 小时处死）或每日一次（最后一次剂量在处死前 24 小时给予，或在第 1、2、4、7 和 14 天进行评估程序）。肌肉愈合通过功能、宏观和组织学进行评估。
未经治疗的挤压腓肠肌复合体对照显示改善有限。6α-甲基强的松龙明显加重了愈合。相比之下，BPC 157 诱导更快的肌肉愈合和功能恢复，并且即使在全身皮质类固醇治疗时腹腔内或局部给予，也能改善肌肉愈合，在所有研究的时间点均表现出功能、宏观和组织学上的改善。
BPC 157 完全逆转了全身皮质类固醇引起的肌肉愈合受损。
