Institute of Pathology, University of Zagreb Medical School, Zagreb, Croatia.
J Physiol Pharmacol. 2009 Dec;60 Suppl 7:191-6.
Angiogenesis is a natural and complex process controlled by angiogenic and angiostatic molecules, with a central role in healing process. One of the most important modulating factors in angiogenesis is the vascular endothelial growth factor (VEGF). Pentadecapeptide BPC 157 promotes healing demonstrating particular angiogenic/angiomodulatory potential. We correlated the angiogenic effect of BPC 157 with VEGF expression using in vitro (cell culture) and in vivo (crushed muscle and transected muscle and tendon) models. Results revealed that there is no direct angiogenic effect of BPC 157 on cell cultures. On the other hand, immunohistochemical analysis of muscle and tendon healing using VEGF, CD34 and FVIII antibodies showed adequately modulated angiogenesis in BPC 157 treated animals, resulting in a more adequate healing. Therefore the angiogenic potential of BPC 157 seems to be closely related to the healing process in vivo with BPC 157 stimulating angiogenesis by up-regulating VEGF expression.
血管生成是一个由血管生成和血管抑制分子控制的自然而复杂的过程，在愈合过程中起着核心作用。血管生成过程中最重要的调节因子之一是血管内皮生长因子（VEGF）。十五肽 BPC 157 具有促进愈合的作用，表现出特殊的血管生成/血管调节潜力。我们使用体外（细胞培养）和体内（肌肉挤压和肌肉肌腱横断）模型，将 BPC 157 的血管生成作用与 VEGF 表达相关联。结果表明，BPC 157 对细胞培养物没有直接的血管生成作用。另一方面，使用 VEGF、CD34 和 FVIII 抗体对肌肉和肌腱愈合进行免疫组织化学分析表明，BPC 157 治疗的动物中血管生成得到了适当的调节，从而实现了更充分的愈合。因此，BPC 157 的血管生成潜力似乎与体内的愈合过程密切相关，BPC 157 通过上调 VEGF 表达刺激血管生成。
