Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Darwin 2, 28049, Madrid, Spain.
Histochem Cell Biol. 2010 Apr;133(4):449-54. doi: 10.1007/s00418-010-0679-9. Epub 2010 Feb 27.
The study of uptake mechanisms of therapeutic drugs has a growing interest in biomedical research. In this work the cell uptake and phototoxicity of the photosensitizer Zn(II)-phthalocyanine (ZnPc) in dipalmitoyl-phosphatidyl-choline liposomes have been studied in the presence or absence of inhibitors of macropinocytosis (cytochalasin D), and clathrin-mediated endocytosis (dynasore). No differences in the uptake or photodynamic damage were observed in A-549 cells subjected to incubation with either ZnPc alone or in combination with cytochalasin D. On the contrary, co-incubation of A-549 cells with ZnPc and dynasore resulted in a significant decrease of photodamage as well as negligible uptake of the photosensitizer. These results indicate that ZnPc is internalized into cells preferentially by a mechanism of clathrin-mediated endocytosis.
治疗药物摄取机制的研究在生物医学研究中越来越受到关注。本工作研究了在存在或不存在巨胞饮抑制剂（细胞松弛素 D）和网格蛋白介导的内吞作用抑制剂（dynasore）的情况下，光敏剂 Zn(II)-酞菁（ZnPc）在二棕榈酰磷脂酰胆碱脂质体中的细胞摄取和光毒性。在用单独的 ZnPc 或与细胞松弛素 D 联合孵育的 A-549 细胞中，观察到摄取或光动力损伤没有差异。相反，当 A-549 细胞与 ZnPc 和 dynasore 共同孵育时，光损伤显著降低，并且光敏剂的摄取可忽略不计。这些结果表明，ZnPc 优先通过网格蛋白介导的内吞作用进入细胞。
