品种系谱中“Marion”黑莓（Rubus sp.）基因型中的结合易变前体。

Bound volatile precursors in genotypes in the pedigree of 'Marion' blackberry (Rubus sp.).

机构信息

Department of Food Science & Technology, Oregon State University, Corvallis, Oregon 97331, USA.

出版信息

J Agric Food Chem. 2010 Mar 24;58(6):3694-9. doi: 10.1021/jf9034089.

PMID:20192206

Abstract

Glycosidically bound volatiles and precursors in genotypes representing the pedigree for 'Marion' blackberry were investigated over two growing seasons. The volatile precursors were isolated using a C18 solid-phase extraction column. After enzymatic hydrolysis, the released volatiles were analyzed using stir bar sorptive extraction gas chromatography-mass spectrometry (GC-MS) and direct microvial insert thermal desorption GC-MS. The most abundant volatile precursors in the genotypes were alcohols, followed by shikimic acid derivatives. High amounts of furanone glycosides were also detected, while norisoprenoids only existed in a small amount in blackberries. The volatile precursor composition in the genotypes in the 'Marion' pedigree was very similar to their free volatile distribution. 'Logan' and 'Olallie' predominantly had bound norisoprenoids. Wild 'Himalaya' predominated with terpene alcohol and furaneol glycosides, whereas 'Santiam' and 'Chehalem' contained a high level of terpene alcohol glycosides. A similar inheritance pattern was also observed for some volatile precursors in the genotypes in the 'Marion' pedigree. A high content of linalool, hydroxylinalool, and alpha-ionol glycosides in 'Olallie' and a low content in 'Chehalem' resulted in a moderate level in their offspring 'Marion', while a low content of (E)-linalool oxide precursor in 'Olallie' and a high content in 'Chehalem' also resulted in a moderate level in 'Marion'. However, the concentration of furaneol glycosides in 'Marion' exceeded that of its two parents.

摘要

在两个生长季节中，研究了代表‘马里恩’黑莓谱系的基因型中糖苷结合的挥发性物质和前体。使用 C18 固相萃取柱分离挥发性前体。在酶解后，使用搅拌棒吸附萃取气相色谱-质谱联用（GC-MS）和直接微管插入热解吸 GC-MS 分析释放的挥发性物质。基因型中最丰富的挥发性前体是醇，其次是莽草酸衍生物。还检测到大量呋喃酮糖苷，而 norisoprenoids 仅在黑莓中少量存在。‘马里恩’谱系中基因型的挥发性前体组成与其游离挥发性分布非常相似。‘洛根’和‘奥拉拉利’主要含有结合的 norisoprenoids。野生的‘喜马拉雅’以萜烯醇和呋喃醇糖苷为主，而‘桑蒂亚姆’和‘切哈勒姆’则含有高水平的萜烯醇糖苷。在‘马里恩’谱系中的基因型中，一些挥发性前体也观察到了类似的遗传模式。‘奥拉拉利’中含有高含量的芳樟醇、羟基芳樟醇和α-依兰醇糖苷，而‘切哈勒姆’中的含量较低，导致其后代‘马里恩’中的含量适中，而‘奥拉拉利’中（E）-芳樟醇氧化物前体含量较低，‘切哈勒姆’中的含量较高，也导致‘马里恩’中的含量适中。然而，呋喃醇糖苷在‘马里恩’中的浓度超过了其两个亲本。