Programa de Posgrado en Alimentos del Centro de la Republica (PROPAC), Universidad Autónoma de Querétaro, Santiago de Querétaro, Querétaro, Mexico.
J Agric Food Chem. 2010 Jun 23;58(12):7458-64. doi: 10.1021/jf101485r.
This study was designed to characterize and compare wild, commercial, and noncommercial cultivated blackberry genotypes grown in Michoacan, Mexico. Six genotypes, including WB-3, WB-7, WB-10, and WB-11 (all wild blackberry types), Tupy (a commercial cultivar), and UM-601 (a cultivated breeding line), were selected and profiled for anthocyanins and proanthocyanidins by separating extracts over Amberlite XAD-7 resin and Sephadex LH-20 columns. Subsequent high-performance liquid chromatography (HPLC) and liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) analyses revealed that the major anthocyanin for all genotypes was cyanidin 3-O-glucoside. The proanthocyanidins (condensed tannins) were present in mono- to hexamer forms. Also, hydrolyzable tannins, ellagitannins, were characterized in the blackberry fruits. The average anthocyanin concentration in Sephadex LH-20 fractions was 49.2 mg/g in the commercial cultivar Tupy, while in the wild genotypes and the breeding line, the range was 361.3-494.9 mg/g (cyanidin 3-O-glucoside equivalent). The proanthocyanidin concentration varied widely among wild genotypes (417.5-1343.6 mg/g, catechin equivalent). This study demonstrated that the use of Amberlite XAD-7 followed by Sephadex LH-20 chromatography, with subsequent HPLC and LC-ESI-MS analyses, was able to effectively separate and characterize the diverse polyphenolics in blackberry genotypes. These results suggest that recommendations for dietary intake of blackberries for human health benefits need to take into account the source, because of the wide inherent variation in bioactive polyphenolic content in different blackberry genotypes.
本研究旨在对在墨西哥米却肯州种植的野生、商业和非商业栽培黑莓基因型进行鉴定和比较。选择了 WB-3、WB-7、WB-10 和 WB-11（均为野生黑莓品种）、Tupy（商业品种）和 UM-601（栽培育种种系）这 6 个基因型，通过用 Amberlite XAD-7 树脂和 Sephadex LH-20 柱分离提取物来对花色苷和原花青素进行分析。随后的高效液相色谱（HPLC）和液相色谱-电喷雾电离质谱（LC-ESI-MS）分析表明，所有基因型的主要花色苷均为矢车菊素 3-O-葡萄糖苷。原花青素（缩合单宁）以单至六聚体的形式存在。此外，在黑莓果实中还鉴定出可水解单宁、鞣花单宁。商业品种 Tupy 在 Sephadex LH-20 级分中的花色苷浓度平均为 49.2mg/g，而在野生基因型和育种种系中，范围为 361.3-494.9mg/g（矢车菊素 3-O-葡萄糖苷当量）。原花青素浓度在野生基因型之间差异很大（417.5-1343.6mg/g，儿茶素当量）。本研究表明，使用 Amberlite XAD-7 后再用 Sephadex LH-20 色谱法，随后进行 HPLC 和 LC-ESI-MS 分析，能够有效地分离和鉴定黑莓基因型中的多种多酚。这些结果表明，为了人类健康益处而推荐食用黑莓时，需要考虑来源，因为不同黑莓基因型中生物活性多酚含量存在广泛的固有差异。
