Xia Yu-gang, Li Yan-ting, Lu Yi-han, Ren Hong, Hu An-qun, Zhu Jian-fu, Wang Xue-cai, Jing Qing-wu, Zheng Ying-jie
Department of Epidemiology, School of Public Health, Fudan University, the Key Laboratory of Public Health Safety, Ministry of Education, Shanghai 200032, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2009 Dec;30(12):1269-72.
To understand phylogenetic characteristics of sporadic hepatitis E virus (HEV) in eastern China.
Four hundred and thirteen sera were collected from sporadic hepatitis E cases in 14 second- or first-class hospitals in Eastern China from 2005 to 2008 and detected with a nested RT-PCR assay. Partial nucleotide sequences of the HEV isolates were determined for phylogenetic analysis with prototype sequences in the GenBank.
The male-to-female sex ratio of the patients was 1.75:1 with 61.5% of them aged 40 - 69 years old. HEV RNA was detected in 140 out of 413(34%)serum samples. Phylogenetic analysis revealed that all the 140 HEV isolates belonged to genotype IV, sharing 77.9% - 88.3%, 80.8% - 90.6%, 73.4% - 85.2% and 91.0% - 95.4% nucleotide sequence identities with prototype I, II, III and IV HEV isolates respectively.
It was evident that genotype IV HEV served as the main causative agent of sporadic HEV infection in Eastern China. However the viral origin and evolution needs further clarification.
了解中国东部地区散发性戊型肝炎病毒（HEV）的系统发育特征。
收集2005年至2008年中国东部14家二级或一级医院散发性戊型肝炎病例的413份血清，采用巢式RT-PCR法进行检测。测定HEV分离株的部分核苷酸序列，与GenBank中的原型序列进行系统发育分析。
患者男女比例为1.75:1，其中61.5%年龄在40 - 69岁之间。413份血清样本中有140份（34%）检测到HEV RNA。系统发育分析表明，所有140株HEV分离株均属于基因型IV，与I、II、III和IV型HEV原型分离株的核苷酸序列同源性分别为77.9% - 88.3%、80.8% - 90.6%、73.4% - 85.2%和91.0% - 95.4%。
显然，IV型HEV是中国东部地区散发性HEV感染的主要病原体。然而，病毒的起源和进化仍需进一步阐明。
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