Xia Yu-gang, Lu Yi-han, Hu An-qun, Qin Xue, Dong Xiao-lian, Zhu Jian-fu, Jiang Qing-wu, Zheng Ying-jie
Department of Epidemiology, School of Public Health, Fudan University, the Key Laboratory of Public Health Safety, Ministry of Education, Shanghai 200032, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2010 Jul;31(7):791-4.
To determine the prevalence and genotype of hepatitis E virus (HEV) among commercial swine population in Eastern and Southern China.
Six hundred specimens of swine bile collected from 5 slaughterhouses in Eastern and Southern China from 2007 to 2009 were tested for HEV RNA using nested RT-PCR. PCR products were sequenced for phylogenetic analysis.
Forty-seven out of the 600 samples (7.83%) were positive for HEV RNA. Based on the 150 nt fragment within HEV ORF2, data from phylogenetic analysis revealed that all the 47 HEV isolates were identified to be genotype IV, sharing 75.0% - 83.4%, 75.0% - 84.6%, 71.9% - 80.7% and 88.1% - 91.5% nucleotide identities with prototype I, II, III and IV HEV strains respectively while majority of the isolates clustered within their respective isolation sites.
HEV was widespread in commercial swine population in Eastern and Southern China that raised a serious concern about the safety regarding the consumption of pork products.
确定中国东部和南部商业猪群中戊型肝炎病毒（HEV）的流行情况和基因型。
采用巢式RT-PCR检测2007年至2009年从中国东部和南部5家屠宰场采集的600份猪胆汁标本中的HEV RNA。对PCR产物进行测序以进行系统发育分析。
600份样本中有47份（7.83%）HEV RNA呈阳性。基于HEV ORF2内的150 nt片段，系统发育分析数据显示，所有47株HEV分离株均被鉴定为IV型，与I、II、III和IV型HEV原型株的核苷酸同一性分别为75.0% - 83.4%、75.0% - 84.6%、71.9% - 80.7%和88.1% - 91.5%，而大多数分离株聚集在各自的分离地点内。
HEV在中国东部和南部的商业猪群中广泛存在，这引发了对猪肉产品消费安全性的严重关注。
