Department of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA.
J Am Coll Surg. 2010 Mar;210(3):271-9. doi: 10.1016/j.jamcollsurg.2009.11.005.
Maintenance of postsurgical tissue oxygenation depends on the ability of the specific tissue to recruit perfusion and oxygen (O(2)) supply. When native O(2)-carrying capacity is lacking, fluids to improve O(2)-carrying capacity based in hemoglobin (Hb) could prevent partially ischemic tissue hypoxia by increasing O(2) release from the remaining red blood cells.
Responses to facilitated O(2) transport after exchange transfusion with polymerized bovine Hb (PBH) were studied in a chronic partially ischemic tissue model, induced by large feeding arteriole ligation during hamster window chamber model implantation. PBH effects in microvascular perfusion and tissue oxygenation were studied after exchange transfusion of 40% of animal's blood volume. Experimental groups were defined by the concentration of PBH used, ie, PBH at 13 g/dL (PBH13); PBH at 4 g/dL in albumin solution to matching colloidal osmotic pressure (COP) (PBH4); and no PBH, only albumin solution at matching COP (PBH0).
Restitution of O(2)-carrying capacity with PBH13 increased blood pressure and produced vasoconstriction compared with PBH4 and PBH0. On the other hand, PBH4 maintained blood pressure without substantial vasoconstriction, increased tissue partial pressure of O(2), arteriolar O(2) supply, and extraction to the partially ischemic tissue compared with PBH0 and PBH13.
Results suggest the existence of an optimal concentration of low O(2)-affinity acellular Hb to increase oxygenation of partially ischemic tissue during anemic conditions.
术后组织氧合的维持取决于特定组织募集灌注和氧（O2）供应的能力。当天然 O2 携带能力不足时，基于血红蛋白（Hb）提高 O2 携带能力的液体可以通过增加剩余红细胞中 O2 的释放来防止部分缺血组织缺氧。
在慢性部分缺血组织模型中研究了聚合牛血红蛋白（PBH）交换输血后促进 O2 转运的反应，该模型通过在仓鼠窗口室模型植入期间结扎大的喂养动脉来诱导。在动物血液体积的 40%进行交换输血后，研究了 PBH 对微血管灌注和组织氧合的影响。实验分组由 PBH 的浓度定义，即 13 g/dL（PBH13）的 PBH；白蛋白溶液中的 4 g/dL PBH 以匹配胶体渗透压（COP）（PBH4）；和没有 PBH，仅匹配 COP 的白蛋白溶液（PBH0）。
与 PBH4 和 PBH0 相比，用 PBH13 恢复 O2 携带能力会增加血压并导致血管收缩。另一方面，与 PBH0 和 PBH13 相比，PBH4 维持血压而没有实质性的血管收缩，增加了部分缺血组织的组织部分压力 O2、动脉供应 O2 和提取。
结果表明，在贫血情况下，增加部分缺血组织氧合存在低 O2 亲和力脱细胞 Hb 的最佳浓度。
