Louw J, Woodroof C W, Wolfe-Coote S A
Medical Research Council, Experimental Biology Programme, Tygerberg, South Africa.
Anat Rec. 1997 Mar;247(3):405-12. doi: 10.1002/(SICI)1097-0185(199703)247:3<405::AID-AR12>3.0.CO;2-T.
Our previous studies on the Chacma baboon revealed that the most striking difference between islets of the ventral and dorsal regions of the pancreas was their content of A and pancreatic polypeptide (PP) cells with A cells predominating in the tail and PP cells in the uncinate and head. Cells displaying dual immunoreactivity for both glucagon and PP were also observed. The objective of this study was to establish baseline parameters of the adult Vervet monkey (Cercopithecus aethiops) pancreas so that it could be used as a primate model to investigate possible therapies for diabetes.
Vervet-monkey pancreas was divided into uncinate, head, and tail regions, and the tissue processed for immunolabelling for pancreatic peptides using avidin-biotin-peroxidase as marker. Dot-blotting and absorption controls for antibody specificity were included because of the shared amino acid sequences in pancreatic polypeptide (PP), neuropeptide Y (NPY), and peptide YY (PYY). Endocrine cell distributions and the percentages of each cell type per region were calculated for each monkey.
A significant difference in the percentages of PP cells (P = 0.02) was observed between uncinate and tail regions, the distribution of NPY cells was similar to that of the PP cells, and all other distributions were similar to those reported in the literature for most animals studied. Cells displaying dual immunoreactivity for glucagon and PP or NPY, PYY and NPY, or PP, PP and somatostatin and glucagon and insulin were identified and mapped throughout the pancreas. Most co-localizations occurred in the uncinate region. Co-localization of glucagon and insulin has not, to our knowledge, been reported previously in the adult pancreas.
Pancreatic endocrine cell distribution in the adult Vervet monkey was found to be very similar to most other animals studied. The occurrence of cells displaying dual immunoreactivity for a number of different combinations of pancreatic peptides suggests an interesting plasticity of endocrine cells even in the adult animal.
我们之前对南非大狒狒的研究表明，胰腺腹侧和背侧区域胰岛之间最显著的差异在于其A细胞和胰多肽（PP）细胞的含量，A细胞在尾部占主导，PP细胞在钩突部和头部占主导。还观察到对胰高血糖素和PP均具有双重免疫反应性的细胞。本研究的目的是建立成年绿猴（非洲长尾猴）胰腺的基线参数，以便将其用作灵长类动物模型来研究糖尿病的可能治疗方法。
将绿猴胰腺分为钩突部、头部和尾部区域，使用抗生物素蛋白-生物素-过氧化物酶作为标记对组织进行处理，以进行胰腺肽的免疫标记。由于胰多肽（PP）、神经肽Y（NPY）和肽YY（PYY）中存在共同的氨基酸序列，因此包括了针对抗体特异性的斑点印迹和吸收对照。计算每只猴子的内分泌细胞分布以及每个区域每种细胞类型的百分比。
在钩突部和尾部区域之间观察到PP细胞百分比存在显著差异（P = 0.02），NPY细胞的分布与PP细胞相似，并且所有其他分布与大多数研究动物的文献报道相似。在整个胰腺中识别并绘制了对胰高血糖素和PP或NPY、PYY和NPY、或PP及PP和生长抑素以及胰高血糖素和胰岛素具有双重免疫反应性的细胞。大多数共定位发生在钩突部区域。据我们所知，胰高血糖素和胰岛素的共定位在成年胰腺中此前尚未见报道。
发现成年绿猴的胰腺内分泌细胞分布与大多数其他研究动物非常相似。对多种不同胰腺肽组合具有双重免疫反应性的细胞的出现表明，即使在成年动物中，内分泌细胞也具有有趣的可塑性。
