Pimpinelli Sergio, Bonaccorsi Silvia, Fanti Laura, Gatti Maurizio
Cold Spring Harb Protoc. 2010 Mar;2010(3):pdb.prot5389. doi: 10.1101/pdb.prot5389.
In this protocol, larval brains from Drosophila are incubated in vitro with colchicine, treated with hypotonic solution, fixed, and squashed in aceto-orcein. This procedure provides a large number of well-spread metaphase figures (200-400 per brain) that can be analyzed for chromosome morphology, the presence of chromosome aberrations, and the degree of ploidy.
在本实验方案中，将果蝇幼虫的大脑在体外与秋水仙碱一起孵育，用低渗溶液处理，固定，然后在醋酸洋红中压片。该程序可提供大量分散良好的中期图像（每个大脑200 - 400个），可用于分析染色体形态、染色体畸变的存在情况以及倍性程度。
