Pimpinelli Sergio, Bonaccorsi Silvia, Fanti Laura, Gatti Maurizio
Cold Spring Harb Protoc. 2010 Mar;2010(3):pdb.prot5390. doi: 10.1101/pdb.prot5390.
The classical chromosome-banding techniques developed for mammalian chromosomes do not differentiate the euchromatic arms of Drosophila mitotic chromosomes. However, some of these techniques produce a sharp and highly reproducible banding of Drosophila heterochromatin. For example, the use of quinacrine-, Hoechst-, and N-banding differentiates Drosophila heterochromatin into 61 cytological entities, allowing precise localization of heterochromatic breakpoints. These banding techniques can also be successfully used to differentiate mitotic heterochromatin of various Drosophila and mosquito species. Here we present protocols routinely used in our laboratories for chromosome banding, including the use of Hoechst, 4',6-diamidino-2-phenylindole (DAPI), quinacrine, and Giemsa stains.
为哺乳动物染色体开发的经典染色体显带技术无法区分果蝇有丝分裂染色体的常染色质臂。然而，其中一些技术能使果蝇异染色质产生清晰且高度可重复的带型。例如，使用喹吖因、Hoechst和N显带可将果蝇异染色质区分为61个细胞学实体，从而实现异染色质断点的精确定位。这些显带技术也可成功用于区分各种果蝇和蚊虫物种的有丝分裂异染色质。在此，我们介绍我们实验室常规用于染色体显带的方法，包括使用Hoechst、4',６-二脒基-2-苯基吲哚（DAPI）、喹吖因和吉姆萨染色剂。
