Lee Soon-Hee, Heo Yong, Kim Young-Chul
Department of Beauty Art, Howon University, Gunsan, Korea.
J Vet Sci. 2010 Mar;11(1):35-41. doi: 10.4142/jvs.2010.11.1.35.
Historically, German chamomile (GC) oil has been used for treatment of skin disorders. BALB/c mice were sensitized twice a week with 100 microL of 1% 2,4-dinitrochlorobenzene (DNCB) and challenged twice the following week with 100 microL of 0.2% DNCB for atopic dermatitis induction. Thereafter, 3% GC oil was applied daily (70 microL, 6 times week) on the dorsal skin for 4 weeks. Saline or jojoba oil was used for the control mice. Blood was collected after second DNCB challenge, and at 2 and 4 weeks after initiating oil application. Serum IgE levels were significantly lowered in the GC oil application group at the end of the 4-week application period. The GC oil application for 4 weeks resulted in reduction in serum IgG1 level compared with that after 2-week application. The GC oil application group showed a significantly lower serum histamine level than the control group 2 weeks after oil application. Scratching frequency of the GC oil application group was significantly lower than either control groups. This study is to demonstrate GC oil's immunoregulatory potential for alleviating atopic dermatitis through influencing of Th2 cell activation.
从历史上看，德国洋甘菊（GC）油一直被用于治疗皮肤疾病。用100微升1%的2,4-二硝基氯苯（DNCB）每周对BALB/c小鼠进行两次致敏，接下来的一周用100微升0.2%的DNCB对其进行两次激发以诱导特应性皮炎。此后，每天在背部皮肤涂抹3%的GC油（70微升，每周6次），持续4周。对照组小鼠使用生理盐水或荷荷巴油。在第二次DNCB激发后以及开始涂抹油剂后的第2周和第4周采集血液。在4周涂抹期结束时，GC油涂抹组的血清IgE水平显著降低。与涂抹2周后相比，涂抹GC油4周导致血清IgG1水平降低。在涂抹油剂2周后，GC油涂抹组的血清组胺水平显著低于对照组。GC油涂抹组的抓挠频率显著低于任何一个对照组。本研究旨在证明GC油通过影响Th2细胞活化来缓解特应性皮炎的免疫调节潜力。
