Ahlstrom Jon D, Erickson Carol A
Department of Molecular and Cellular Biology, University of California, Davis, Davis, CA, USA.
Commun Integr Biol. 2009 Nov;2(6):489-93. doi: 10.4161/cib.2.6.9406.
By developing a technique for imaging the avian neural crest epithelial-mesenchymal transition (EMT), we have discovered cellular behaviors that challenge current thinking on this important developmental event, including the probability that complete disassembly of the adherens junctions may not control whether or not a neural epithelial cell undergoes an EMT. Further, neural crest cells can adopt multiple modes of cell motility in order to emigrate from the neuroepithelium. We also gained insights into interkinetic nuclear migration (INM). For example, the movement of the nucleus from the basal to apical domain may not require microtubule motors nor an intact nuclear envelope, and the nucleus does not always need to reach the apical surface in order for cytokinesis to occur. These studies illustrate the value of live-cell imaging to elucidate cellular processes.
通过开发一种对鸟类神经嵴上皮-间充质转化（EMT）进行成像的技术，我们发现了一些细胞行为，这些行为挑战了目前关于这一重要发育事件的认知，包括黏附连接的完全解体可能并不控制神经上皮细胞是否经历EMT的可能性。此外，神经嵴细胞可以采用多种细胞运动模式以便从神经上皮中迁出。我们还对细胞周期中核迁移（INM）有了深入了解。例如，细胞核从基底区域向顶端区域的移动可能不需要微管马达也不需要完整的核膜，并且为了发生胞质分裂细胞核并不总是需要到达顶端表面。这些研究说明了活细胞成像在阐明细胞过程方面的价值。
