Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Arch Virol. 2010 Apr;155(4):491-8. doi: 10.1007/s00705-010-0608-1. Epub 2010 Mar 2.
Human influenza A virus (IAV) is a major cause of life-threatening respiratory tract disease worldwide. Defensins are small cationic peptides of about 2-6 kDa that are known for their broad-spectrum antimicrobial activity. Here, we focused on the anti-influenza A activity of mouse beta-defensin 2 (mBD2). The prokaryotic expression plasmid pET32a-mBD2 was constructed and introduced into Escherichia coli Rosseta gami (2) to produce recombinant mBD2 (rmBD2). Purified rmBD2 showed strong antiviral activity against IAV in vitro. The protective rate for Madin-Darby canine kidney cells was 93.86% at an rmBD2 concentration of 100 microg/ml. Further studies demonstrated that rmBD2 prevents IAV infection by inhibiting viral entry. In addition, both pretreatment and postinfection treatment with rmBD2 provided protection against lethal virus challenge with IAV in experimental mice, with protection rates of 70 and 30%, respectively. These results suggest that the mBD2 might have important effects on influenza A virus invasion.
人甲型流感病毒（IAV）是全球范围内导致危及生命的呼吸道疾病的主要原因。防御素是一种约 2-6 kDa 的小阳离子肽，以广谱抗菌活性而闻名。在这里，我们专注于研究小鼠β-防御素 2（mBD2）的抗甲型流感病毒活性。构建了原核表达质粒 pET32a-mBD2，并将其导入大肠杆菌 Rosetta gami（2）中生产重组 mBD2（rmBD2）。纯化的 rmBD2 在 100 μg/ml 的浓度下表现出对 IAV 的强烈体外抗病毒活性。rmBD2 对 Madin-Darby 犬肾细胞的保护率为 93.86%。进一步的研究表明，rmBD2 通过抑制病毒进入来阻止 IAV 感染。此外，rmBD2 预处理和感染后处理均可分别为实验小鼠提供针对致死性病毒攻击的保护，保护率分别为 70%和 30%。这些结果表明，mBD2 可能对甲型流感病毒入侵具有重要影响。
