Hirsch V M, Zack P M, Vogel A P, Johnson P R
Department of Microbiology, Georgetown University, Maryland.
J Infect Dis. 1991 May;163(5):976-88. doi: 10.1093/infdis/163.5.976.
Four simian immunodeficiency virus (SIV)-infected macaques in the terminal stages of AIDS were studied. Easily detectable unintegrated proviral DNA was present in nearly every tissue (lymphoid and nonlymphoid) examined. SIV-specific immunohistochemistry revealed that macrophages or macrophage-like cells were the predominant reservoirs for SIV. High burdens of infectious SIV were also characteristic of end-stage disease. At the molecular level, sequence analyses of molecular clones derived directly from tissue demonstrated that SIV, like other RNA viruses, exists as a mixture of closely related genomes. Viruses derived by cocultivation seemed to be selected for their ability to grow in tissue culture and did not represent the wide spectrum of genotypes in tissues. These data indicate that SIV-induced immunodeficiency is a complex, multisystem disease and that SIV replication, in addition to impairing the immune system, may have direct deleterious effects on multiple organ systems.
对4只处于艾滋病末期的感染了猿猴免疫缺陷病毒（SIV）的猕猴进行了研究。在几乎每一个检测的组织（淋巴组织和非淋巴组织）中都存在易于检测到的未整合前病毒DNA。SIV特异性免疫组织化学显示，巨噬细胞或巨噬样细胞是SIV的主要储存库。高负荷的感染性SIV也是终末期疾病的特征。在分子水平上，直接从组织中获得的分子克隆的序列分析表明，SIV与其他RNA病毒一样，以密切相关基因组的混合物形式存在。通过共培养获得的病毒似乎因其在组织培养中的生长能力而被选择，并不代表组织中广泛的基因型。这些数据表明，SIV诱导的免疫缺陷是一种复杂的多系统疾病，并且SIV复制除了损害免疫系统外，可能还对多个器官系统有直接的有害影响。
