Hirsch V M, Zack P M, Johnson P R
Department of Microbiology, Georgetown University School of Medicine, Rockville, MD.
J Med Primatol. 1990;19(3-4):287-94.
Tissues from SIV-infected, immunodeficient macaques were analyzed by southern blot and polymerase chain reaction (PCR) amplification of env sequences. Provirus was readily detected in both lymphoid and non-lymphoid tissues by southern blot analysis. The majority of virus was in an unintegrated state. Analysis of envelope sequences by PCR revealed that tissues contain many distinct, but closely related genotypes; however, only a subset of these proviral genomes are recovered after tissue culture passage in human cell lines. Thus, tissue culture isolates of SIV do not represent the complete spectrum of genotypes in an infected macaque.
通过Southern印迹法以及env序列的聚合酶链反应（PCR）扩增，对感染SIV的免疫缺陷猕猴的组织进行了分析。通过Southern印迹分析，在淋巴组织和非淋巴组织中均很容易检测到前病毒。大多数病毒处于未整合状态。通过PCR对包膜序列进行分析表明，组织中含有许多不同但密切相关的基因型；然而，在人细胞系中进行组织培养传代后，仅能回收这些前病毒基因组的一个子集。因此，SIV的组织培养分离株并不代表感染猕猴中基因型的完整谱系。
