He Tao, Feng Guihai, Chen Huipeng, Wang Li, Wang Yumin
Beijing Institute of Biotechnology, Beijing 100071, China.
Bioinformation. 2009 Sep 30;4(3):112-8. doi: 10.6026/97320630004112.
The discovery of microRNAs (miRNAs) is a remarkable breakthrough in the field of life science, and they are important actors which regulate gene expression in diverse cellular processes. Recently, several reports indicated that miRNAs can also target viruses and regulate virus replication. Here we discovered 36 pig-encoded miRNAs and 22 human-encoded miRNAs which have putative targets in swine influenza virus (SIV) and Swine-Origin 2009 A/H1N1 influenza virus (S-OIV) genes respectively. Interestingly, the putative interactions of ssc-miR-124a, ssc-miR-136 and ssc-miR-145 with their SIV target genes had been found to be maintained almost throughout all of the virus evolution. Enrichment analysis of previously reported miRNA gene expression profiles revealed that three miRNAs are expressed at higher levels in human lung or trachea tissue. The hsa-miR-145 and hsa-miR-92a putatively target the HA gene and hsa-miR-150 putatively targets the PB2 gene. Analysis results based on the location distribution from which virus was isolated and sequence conservation imply that some putative miRNA-mediated host-virus interactions may characterize the location-specificity.
微小RNA（miRNA）的发现是生命科学领域一项非凡的突破，它们是在多种细胞过程中调节基因表达的重要因子。最近，有几份报告表明，miRNA也可以靶向病毒并调节病毒复制。在此，我们发现了36种猪编码的miRNA和22种人编码的miRNA，它们分别在甲型流感病毒（SIV）和2009年甲型H1N1流感病毒（S-OIV）基因中具有假定的靶标。有趣的是，已发现猪miR-124a、猪miR-136和猪miR-145与其SIV靶基因之间的假定相互作用几乎在整个病毒进化过程中都得以维持。对先前报道的miRNA基因表达谱的富集分析表明，三种miRNA在人肺或气管组织中表达水平较高。人miR-145和人miR-92a可能靶向血凝素（HA）基因，人miR-150可能靶向聚合酶基本蛋白2（PB2）基因。基于病毒分离位置分布和序列保守性的分析结果表明，一些假定的miRNA介导的宿主-病毒相互作用可能具有位置特异性特征。
