Jamwal Vishawdeep Singh, Modi Gourav, George Aman, Chauhan Manmohan Singh
Embryo Biotechnology Lab, Animal Biotechnology Centre, National Dairy Research Institute, Karnal-132001, Haryana, India.
Bioinformation. 2009 Oct 13;4(4):155-7. doi: 10.6026/97320630004155.
Interferon (IFN)-tau secreted only by ruminant endometrium, helps in maternal recognition of pregnancy and exhibit antiviral and antiproliferative activity. Among different types of IFN-tau, IFN-tau1c and IFN- tau3a are the most highly expressed isoforms. In the present study structure of INF-tau1c was predicted using homology modelling. The best model was selected based on overall stereo-chemical quality. The generated 3D structure of the Interferon-tau1c protein of Bos taurus was predicted using the ovine interferon-tau (PDB ID: 1B5L_A) as template. The structure comprises of 5 alpha helices separated by loop regions, which is similar to the one predicted for other IFNs. Molecular interactions of bovine IFN-tau1c with human interferon Type 1 receptor (IFNAR1) was explored in an attempt to predict human IFNAR1 binding sites of IFN-tau1c.
仅由反刍动物子宫内膜分泌的干扰素（IFN）-τ有助于母体识别妊娠，并具有抗病毒和抗增殖活性。在不同类型的IFN-τ中，IFN-τ1c和IFN-τ3a是表达量最高的亚型。在本研究中，使用同源建模预测了INF-τ1c的结构。基于整体立体化学质量选择了最佳模型。以绵羊干扰素-τ（PDB ID：1B5L_A）为模板，预测了牛干扰素-τ1c蛋白的三维结构。该结构由5个α螺旋组成，中间由环区隔开，这与其他干扰素的预测结构相似。为了预测IFN-τ1c的人1型干扰素受体（IFNAR1）结合位点，研究了牛IFN-τ1c与人IFNAR1的分子相互作用。
