Jang Hwanhee, Choi Yohan, Yoo Inkyu, Han Jisoo, Kim Minjeong, Ka Hakhyun
Division of Biological Science and Technology and IPAID, Yonsei University, Wonju, Republic of Korea.
Division of Biological Science and Technology and IPAID, Yonsei University, Wonju, Republic of Korea.
Theriogenology. 2017 Jan 15;88:166-173. doi: 10.1016/j.theriogenology.2016.09.025. Epub 2016 Sep 22.
Type I interferons (IFNs) bind to the heterodimeric receptor composed of IFN-α/β receptor 1 (IFNAR1) and IFN-α and β receptor 2 (IFNAR2) to transmit signals into the cell. It is well known that IFN-δ (IFND), a type I IFN, is secreted by the conceptus during early pregnancy in pigs. However, expression and regulation of IFNAR1 and IFNAR2 in the porcine uterine endometrium are not well understood. Thus, we analyzed the expression and regulation of IFNAR1 and IFNAR2 in the uterine endometrium during the estrous cycle and pregnancy and conceptus and chorioallantoic tissues during pregnancy in pigs. The IFNAR1 and IFNAR2 mRNAs were expressed in the uterine endometrium, and their levels on Day 12 of pregnancy were higher than those on Day 12 of the estrous cycle and highest during pregnancy. The IFNAR1 and IFNAR2 mRNAs were also expressed in conceptuses during early pregnancy, in chorioallantoic tissues during mid-to-term pregnancy, and in endometrial epithelial cells and chorionic membrane during mid-to-late pregnancy. The abundance of IFNAR1 and IFNAR2 mRNAs was increased by interleukin-1β (IL1B), and the abundance of IFNAR2 was increased by estradiol in endometrial tissue explants. Thus, IFNAR1 and IFNAR2 mRNAs were expressed in the uterine endometrium during the estrous cycle and pregnancy in a pregnancy status- and stage-specific manner, and their expression was affected by estradiol and/or IL1B. These results suggest that endometrial and conceptus IFNAR1 and IFNAR2 may mediate the action of type I IFNs during the implantation period for the establishment and maintenance of pregnancy in pigs.
I型干扰素（IFN）与由IFN-α/β受体1（IFNAR1）和IFN-α及β受体2（IFNAR2）组成的异二聚体受体结合，从而将信号传递到细胞内。众所周知，I型干扰素IFN-δ（IFND）由猪妊娠早期的孕体分泌。然而，猪子宫内膜中IFNAR1和IFNAR2的表达及调控情况尚不清楚。因此，我们分析了猪发情周期、妊娠期间子宫内膜中IFNAR1和IFNAR2的表达及调控情况，以及妊娠期间孕体和绒毛尿囊组织中IFNAR1和IFNAR2的表达及调控情况。IFNAR1和IFNAR2 mRNA在子宫内膜中表达，妊娠第12天其水平高于发情周期第12天，且在妊娠期间最高。IFNAR1和IFNAR2 mRNA在妊娠早期的孕体、妊娠中期至晚期的绒毛尿囊组织以及妊娠中期至晚期的子宫内膜上皮细胞和绒毛膜中也有表达。在子宫内膜组织外植体中，白细胞介素-1β（IL1B）可增加IFNAR1和IFNAR2 mRNA的丰度，雌二醇可增加IFNAR2的丰度。因此，IFNAR1和IFNAR2 mRNA在猪发情周期和妊娠期间的子宫内膜中以妊娠状态和阶段特异性方式表达，其表达受雌二醇和/或IL1B影响。这些结果表明，子宫内膜和孕体中的IFNAR1和IFNAR2可能在猪着床期介导I型干扰素的作用，以建立和维持妊娠。
