电化学 DNA 三明治法检测，用脂肪酶标记物进行 DNA 的 attomole 检测。
Electrochemical DNA sandwich assay with a lipase label for attomole detection of DNA.
机构信息
Centre for DNA Nanotechnology, Department of Chemistry and iNANO, Aarhus University, Langelandsgade 140, DK-8000 Aarhus C, Denmark.
出版信息
Chem Commun (Camb). 2010 Mar 21;46(11):1836-8. doi: 10.1039/b924627d. Epub 2010 Feb 12.
DOI:10.1039/b924627d
PMID:20198225
Abstract
A fast and sensitive electrochemical lipase-based sandwich hybridization assay for detection of attomole levels of DNA has been developed. A combination of magnetic beads, used for pre-concentration and bioseparation of the analyte with a lipase catalyst label allowed detection of DNA with a limit of 20 amol.
摘要
已经开发出一种快速灵敏的基于电化学脂肪酶的夹心杂交分析方法，用于检测纳摩尔级别的 DNA。通过将磁性珠与脂肪酶催化剂标签结合使用，实现了对分析物的预浓缩和生物分离，从而能够检测到 20 飞摩尔的 DNA。
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