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基于电化学免疫和适配体的细菌检测方法:与传统检测方案相比的优缺点

Electrochemical Immuno- and Aptamer-Based Assays for Bacteria: Pros and Cons over Traditional Detection Schemes.

作者信息

Jamal Rimsha Binte, Shipovskov Stepan, Ferapontova Elena E

机构信息

Interdisciplinary Nanoscience Center (iNANO), Aarhus University Gustav Wieds Vej 14, DK-8000 Aarhus, Denmark.

出版信息

Sensors (Basel). 2020 Sep 28;20(19):5561. doi: 10.3390/s20195561.

DOI:10.3390/s20195561
PMID:32998409
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7582323/
Abstract

Microbiological safety of the human environment and health needs advanced monitoring tools both for the specific detection of bacteria in complex biological matrices, often in the presence of excessive amounts of other bacterial species, and for bacteria quantification at a single cell level. Here, we discuss the existing electrochemical approaches for bacterial analysis that are based on the biospecific recognition of whole bacterial cells. Perspectives of such assays applications as emergency-use biosensors for quick analysis of trace levels of bacteria by minimally trained personnel are argued.

摘要

人类环境的微生物安全性和健康需要先进的监测工具,既用于在复杂生物基质中特异性检测细菌(通常存在大量其他细菌种类),也用于在单细胞水平上对细菌进行定量。在此,我们讨论基于对完整细菌细胞进行生物特异性识别的现有电化学细菌分析方法。论证了此类检测方法作为应急生物传感器的应用前景,可供训练不足的人员快速分析痕量水平的细菌。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d25/7582323/af003c1980c3/sensors-20-05561-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d25/7582323/f984b55cbab6/sensors-20-05561-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d25/7582323/135e4d4db34b/sensors-20-05561-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d25/7582323/af003c1980c3/sensors-20-05561-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d25/7582323/f984b55cbab6/sensors-20-05561-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d25/7582323/135e4d4db34b/sensors-20-05561-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1d25/7582323/af003c1980c3/sensors-20-05561-g003.jpg

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