Saarland University Medical Center, Department of Internal Medicine, Division of Immunotherapy and Gene Therapy, José Carreras Research Center, D-66421 Homburg/Saar, Germany.
Int J Oncol. 2010 Apr;36(4):955-60. doi: 10.3892/ijo_00000574.
Sirtuins are critical players within multiple cellular pathways such as stress response, apoptosis and energy metabolism. They are associated with metabolic and degenerative diseases, the pathogenesis of cancer and are key elements in the regulation of cellular life span. From within the 7 known human sirtuins, SIRT3 recently stepped out of the shadow of SIRT1 showing strong effects on stress response, apoptosis, cell cycle and energy metabolism, mimicking effects of caloric restriction. We have identified two non-synonymous human SIRT3 SNPs and evaluated their impact on SIRT3 activity and stability. We assessed their influence on cellular energy metabolism in relation to SIRT1 and identified SIRT3 to increase cellular respiration by 80% when compared to SIRT1, which increased cellular respiration by only 30%.
Sirtuins 是多种细胞途径中的关键参与者，如应激反应、细胞凋亡和能量代谢。它们与代谢和退行性疾病、癌症的发病机制有关，是调节细胞寿命的关键因素。在已知的 7 个人类 Sirtuins 中，SIRT3 最近从 SIRT1 的阴影中走出来，对应激反应、细胞凋亡、细胞周期和能量代谢表现出强烈的影响，模拟了热量限制的效果。我们已经确定了两个人类 SIRT3 的非同义 SNP，并评估了它们对 SIRT3 活性和稳定性的影响。我们评估了它们与 SIRT1 相关的对细胞能量代谢的影响，并发现 SIRT3 与 SIRT1 相比，将细胞呼吸增加了 80%，而 SIRT1 仅增加了 30%。
