Institute of Molecular Biology and Genetics of NASU, 150 Zabolotnogo Str., 03143, Kyiv, Ukraine.
J Fluoresc. 2010 Jul;20(4):865-72. doi: 10.1007/s10895-010-0630-4. Epub 2010 Mar 3.
Spectral-fluorescent properties of benzothiazole styryl monomer (Bos-3) and homodimer (DBos-21) dyes in presence of DNA were studied. The dyes enhance their fluorescence intensity in 2-3 orders of magnitude upon interaction with DNA. Studied styrylcyanines in DNA presence demonstrate rather high values of two-photon absorption (TPA) cross-section, which are comparable with the values of TPA cross section of the rhodamine dyes. An applicability of the styrylcyanines as probes for the fluorescence microscopy of living cells was studied. It was shown that both dyes are cell-permeable but homodimer dye DBos-21 produces noticeably brighter staining of HeLa cells comparing with monomer dye Bos-3. Molecules of DBos-21 initially bind to the nucleic acids-containing cell organelles (presumable mitochondria) and are able to penetrate into the cell nucleus. Thus, homodimer styryl DBos-21 dye is viewed as efficient stain for single-photon and two-photon excitation fluorescence imaging of living cells.
研究了苯并噻唑苯乙烯单体（Bos-3）和同二聚体（DBos-21）染料在 DNA 存在下的光谱荧光性质。这些染料与 DNA 相互作用后，其荧光强度增强了 2-3 个数量级。在 DNA 存在下研究的苯乙烯氰染料表现出相当高的双光子吸收（TPA）截面值，与罗丹明染料的 TPA 截面值相当。研究了苯乙烯氰作为活细胞荧光显微镜探针的适用性。结果表明，两种染料均具有细胞通透性，但与单体染料 Bos-3 相比，同二聚体染料 DBos-21 对 HeLa 细胞的染色明显更亮。DBos-21 分子最初与含有核酸的细胞器（可能是线粒体）结合，并能够穿透入细胞核。因此，同二聚体苯乙烯 DBos-21 染料被视为活细胞单光子和双光子激发荧光成像的有效染色剂。
