微卫星基因座从外来鹿科动物转移至巴西小颈鹿（南美泽鹿属，哺乳纲：鹿科）的可转移性

Transferability of microsatellite loci from exotic Cervidae to Brazilian brocket deer (Mazama spp, Mammalia: Cervidae).

作者信息

Mantellatto A M B, Carnelossi E A G, Duarte J M B

机构信息

Departamento de Zootecnia, Núcleo de Pesquisa e Conservação de Cervídeos, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, Universidade Estadual Paulista, Jaboticabal, SP, Brasil.

出版信息

Genet Mol Res. 2010 Feb 18;9(1):277-82. doi: 10.4238/vol9-1gmr720.

DOI:10.4238/vol9-1gmr720

PMID:20198583

Abstract

Transferability of microsatellite loci between closely related species has been reported in several species. This helps reduce costs involved with the development of primers for newly investigated species. Fifteen microsatellite primers developed for Rangifer tarandus, Cervus elaphus, C. axis, and Moschus berezovskii were tested on five species of Brazilian brocket deer of the genus Mazama (M. americana, M. bororo, M. gouazoubira, M. nana, and M. nemorivaga). These primers were tested with DNA extracted from blood samples of two individuals of each species obtained from the Núcleo de Pesquisa e Conservação de Cervídeos (NUPECCE) of Universidade Estadual Paulista (UNESP). Fourteen of the 15 primers tested amplified microsatellite regions of all five species of Mazama, confirmed by sequencing of the amplified fragments. We conclude that these primers could be used for population studies of brocket deer.

摘要

在几个物种中都报道了微卫星基因座在亲缘关系密切的物种之间的可转移性。这有助于降低为新研究物种开发引物所涉及的成本。为驯鹿、马鹿、梅花鹿和黑麝开发的15个微卫星引物在巴西短角鹿属的5个物种（美洲短角鹿、博罗罗短角鹿、南美泽鹿、倭短角鹿和林短角鹿）上进行了测试。这些引物用从圣保罗州立大学（UNESP）的鹿类研究与保护中心（NUPECCE）获得的每个物种的两个个体的血液样本中提取的DNA进行测试。测试的15个引物中有14个扩增出了所有5种短角鹿的微卫星区域，扩增片段测序证实了这一点。我们得出结论，这些引物可用于短角鹿的种群研究。