Lin D Y, Chiang T Y, Huang C C, Lin H D, Tzeng S J, Kang S R, Sung H M, Wu M C
Department of Life Sciences, Cheng Kung University, Tainan, Taiwan
Department of Life Sciences, Cheng Kung University, Tainan, Taiwan.
Genet Mol Res. 2014 May 23;13(2):3967-71. doi: 10.4238/2014.May.23.7.
Primers for eight microsatellites were developed; they successfully amplified DNA from 20 domesticated Formosan Sambar deer (Cervus unicolor swinhoei). All loci were polymorphic, with 10-19 alleles per locus. The average observed heterozygosity across loci and samples was 0.310, ranging from 0 to 0.750 at each locus. All loci but one, CU18, deviated from Hardy-Weinberg equilibrium due to excessive homozygosity in these domesticated broodstocks, reflecting inbreeding. These microsatellite loci will be useful, not only for assessment of population structure and genetic variability, but also for conservation of wild deer populations in Taiwan.
开发了用于八个微卫星的引物；它们成功地从20只圈养的台湾水鹿（Cervus unicolor swinhoei）中扩增出了DNA。所有位点均具有多态性，每个位点有10 - 19个等位基因。各位点和样本的平均观察杂合度为0.310，每个位点的杂合度范围为0至0.750。除了CU18位点外，所有位点均因这些圈养亲鱼中纯合度过高而偏离哈迪 - 温伯格平衡，这反映了近亲繁殖。这些微卫星位点不仅将有助于评估种群结构和遗传变异性，还将有助于台湾野生鹿种群的保护。
