Andrianova B V, Goriacheva I I, Aleksandrov I D, Gorelova T V
Genetika. 2010 Jan;46(1):14-7.
Wolbachia pipientis is an obligately intracellular bacterium infecting a number of arthropod and nematode species. At the body level, Wolbachia infection may cause parthenogenesis, feminization of genetic males, male killing, or cytoplasmic incompatibility; it may also be asymptomatic. Of special interest is DNA transfer from Wolbachia to the host insect genome, which was discovered recently. At the cellular level, the effects caused by Wolbachia have been studied more poorly. Only one of the known insect cell lines has been obtained from an insect species (the mosquito Aedes albopictus) infected by Wolbachia. In this study, a continuous cell line Dm2008Wb1 has been obtained from embryos of Drosophila melanogaster infected under natural conditions. Wolbachia both persists in a primary cell culture and is retained upon its transformation into a continuous culture. The presence of this bacterium in cells in a free form is evidenced by the fact that tetracycline treatment can cure the cells of Wolbachia and by successful transfer of Wolbachia to another cell line (S2), where it has not been detected before.
嗜虫沃巴赫氏体是一种专性细胞内细菌，可感染多种节肢动物和线虫物种。在个体层面，沃巴赫氏体感染可能导致孤雌生殖、遗传雄性的雌性化、雄性致死或细胞质不亲和；也可能没有症状。最近发现的沃巴赫氏体向宿主昆虫基因组的DNA转移尤其令人感兴趣。在细胞层面，对沃巴赫氏体所造成影响的研究较少。已知的昆虫细胞系中只有一个是从感染了沃巴赫氏体的昆虫物种（白纹伊蚊）获得的。在本研究中，从在自然条件下感染的黑腹果蝇胚胎中获得了一个连续细胞系Dm2008Wb1。沃巴赫氏体在原代细胞培养中持续存在，并在其转化为连续培养时得以保留。四环素处理可使细胞清除沃巴赫氏体，以及成功地将沃巴赫氏体转移到另一个之前未检测到它的细胞系（S2），这些事实证明了这种细菌以游离形式存在于细胞中。
