Mogil'naia O A, Puzyr' A P, Bondar' V S
Prikl Biokhim Mikrobiol. 2010 Jan-Feb;46(1):40-4.
The effect of aflatoxin B1 on growth and luminescence of marine luminous bacteria P. phosphoreum and recombinant E. coli Z905 cells was investigated. The bidirectional effect of aflatoxin B1 on the studied bacterial species was detected--an inhibition of luminescence in P. phosphoreum and its stimulation in E. coli. It was shown that aflatoxin B1 influences the cell luminescence in the freshly grown cultures and bacteria restored after lyophilization. It was detected that the effect of aflatoxin B1 was graded after interaction with the modified nanodiamond (MND) of detonation synthesis. After mycotoxin's treatment with MND, it does not cause significant changes in bacterial luminescence. The possibilities for the use of P. phosphoreum and E. coli bacteria in the bioluminescent monitoring of aflatoxin B1 and the use of MND for mycotoxin deactivation are discussed.
研究了黄曲霉毒素B1对海洋发光细菌发光杆菌（Photobacterium phosphoreum）和重组大肠杆菌Z905细胞生长及发光的影响。检测到黄曲霉毒素B1对所研究细菌具有双向作用——抑制发光杆菌的发光，而刺激大肠杆菌的发光。结果表明，黄曲霉毒素B1对新鲜培养的细胞以及冻干后复苏的细菌的发光均有影响。检测发现，黄曲霉毒素B1与爆轰合成的改性纳米金刚石（MND）相互作用后，其作用呈梯度变化。用MND处理霉菌毒素后，其不会引起细菌发光的显著变化。讨论了利用发光杆菌和大肠杆菌进行黄曲霉毒素B1生物发光监测以及利用MND使霉菌毒素失活的可能性。
