Deriabin D G, Karimov I F
Prikl Biokhim Mikrobiol. 2010 Jan-Feb;46(1):35-9.
It was found that divalent iron ions have alternative effects on the bioluminescence of the natural marine microorganism Photobacterium phosphoreum and the recombinant Escherichia coli strain with a cloned lux operon of P. leiognathi. In the presence of 0.25-5.0 mM FeSO4, the bioluminescence intensity of the former and the latter increased and decreased, respectively. To establish the causes of these differences, we studied the characteristics of the fatty acid composition of the compared microorganisms. The fatty acid profile of E. coli was characterized by a high proportion of unsaturated 11-octadecenoic (vaccenic) acid. A study of this acid in a cell-free enzyme system used for bioluminescence generation showed that it is a potent inhibitor of bacterial bioluminescence. It was found that such effects are enhanced if 11-octadecenoid acid is preincubated with Fe2+.
研究发现，二价铁离子对天然海洋微生物费氏弧菌以及带有克隆的利氏弧菌lux操纵子的重组大肠杆菌菌株的生物发光具有不同影响。在存在0.25 - 5.0 mM硫酸亚铁的情况下，前者和后者的生物发光强度分别增加和降低。为了确定这些差异的原因，我们研究了所比较微生物的脂肪酸组成特征。大肠杆菌的脂肪酸谱以高比例的不饱和11 - 十八碳烯酸（反油酸）为特征。在用于产生生物发光的无细胞酶系统中对这种酸的研究表明，它是细菌生物发光的有效抑制剂。研究发现，如果11 - 十八碳烯酸与Fe2 +预孵育，这种影响会增强。
