Mishutkina Ia V, Kamionskaia A M, Skriabin K G
Prikl Biokhim Mikrobiol. 2010 Jan-Feb;46(1):89-95.
The parameters of transformation using Agrobacterium tumefaciens EHA 105 for 5 domestic sorts and lines of sugar beet (Beta vulgaris L. var. saccharifera (Alef) Krass) were optimized. The system of transgenic tissue selection based on resistance to phosphinothricin, allowing to avoid the appearing of chimeric shoots among initial transformants was developed. The transgenic plants of sugar beet sorts Ramonskaya single seed 47, L'govskaya single seed 52 and RMS 73, and LBO 17 and LBO 19 lines expressing the gene of phosphinothricin acetyl transferase bar have been obtained. The resistance of these sorts and lines to the effect of phosphinothricin in vitro has been shown.
对根癌农杆菌EHA 105转化5个国内甜菜（Beta vulgaris L. var. saccharifera (Alef) Krass）品种和品系的参数进行了优化。建立了基于对草丁膦抗性的转基因组织筛选系统，可避免初始转化体中出现嵌合芽。已获得表达草丁膦乙酰转移酶bar基因的甜菜品种拉蒙斯卡亚单粒47、利戈夫斯卡亚单粒52和RMS 73，以及LBO 17和LBO 19品系的转基因植株。已证明这些品种和品系在体外对草丁膦作用具有抗性。
