Hormones and Signal Transduction Group, German Cancer Research Centre, DKFZ-ZMBH Alliance, Im Neuenheimer Feld 581, Heidelberg, Germany.
Arch Physiol Biochem. 2010 May;116(2):73-8. doi: 10.3109/13813451003631439.
Insulin glargine (Lantus) stimulates growth of MCF-7 cells stronger than human insulin. We investigated if serum from diabetic patients treated with glargine versus human insulin may display a similar effect.
Pairs of serum samples from 31 C-peptide negative type-1 diabetic patients were investigated. In cross-over fashion, 23 patients were treated with glargine plus rapid-acting insulin analogues, and similar doses of human NPH and rapid-acting insulin. For comparison, eight patients were treated with insulin detemir (Levemir) and human NPH. MCF-7 cells were incubated with 10% serum and proliferation was assessed after 72 hours.
Serum containing insulin glargine was 1.11(95% CI 1.05-1.18) fold more mitogenic than human insulin-containing serum (p < 0.005); mitogenicity of serum containing detemir was 0.99(95% CI 0.98-1.02) fold that of human insulin-containing serum.
The serum of diabetic patients was slightly stronger mitogenic when using glargine as compared to human insulin or detemir for treatment.
甘精胰岛素（来得时）比人胰岛素更能刺激 MCF-7 细胞的生长。我们研究了用甘精胰岛素和人胰岛素治疗的糖尿病患者的血清是否可能显示出类似的作用。
对 31 名 C 肽阴性 1 型糖尿病患者的配对血清样本进行了研究。在交叉设计中，23 名患者接受甘精胰岛素加速效胰岛素类似物和人 NPH 及速效胰岛素的类似剂量治疗。为了比较，8 名患者接受了地特胰岛素（来得时）和人 NPH 的治疗。用 10%的血清孵育 MCF-7 细胞，72 小时后评估增殖情况。
含有甘精胰岛素的血清比含有人胰岛素的血清具有 1.11 倍（95%CI 1.05-1.18；p<0.005）的更强的促有丝分裂作用；含有地特胰岛素的血清的促有丝分裂作用是人胰岛素的 0.99 倍（95%CI 0.98-1.02）。
与用人胰岛素或地特胰岛素治疗相比，糖尿病患者的血清在用甘精胰岛素治疗时具有稍微更强的促有丝分裂作用。
