Department of Conservative Dentistry, School of Dentistry, Kyung Hee University, Seoul, Korea.
J Dent Res. 2010 Jun;89(6):575-80. doi: 10.1177/0022034510363380. Epub 2010 Mar 3.
During mineralization, unbound water within the collagen matrix is replaced by apatite. This study tested the null hypothesis that there is no difference in the status of in vitro biomimetic remineralization of hybrid layers, regardless of their moisture contents. Acid-etched dentin was bonded with One-Step with ethanol-wet-bonding, water-wet-bonding, and water-overwet-bonding protocols. Composite-dentin slabs were subjected to remineralization for 1-4 months in a medium containing dual biomimetic analogs, with set Portland cement as the calcium source and characterized by transmission electron microscopy. Remineralization was either non-existent or restricted to the intrafibrillar mode in ethanol-wet-bonded specimens. Extensive intrafibrillar and interfibrillar remineralization was observed in water-wet-bonded specimens. Water-overwet specimens demonstrated partial remineralization of hybrid layers and precipitation of mineralized plates within water channels. The use of ethanol-wet-bonding substantiates that biomimetic remineralization is a progressive dehydration process that replaces residual water in hybrid layers with apatite crystallites.
在矿化过程中，胶原基质中的未结合水被磷灰石取代。本研究检验了一个零假设，即无论其含水量如何，混合层的体外仿生再矿化状态都没有差异。酸蚀牙本质用 One-Step 与乙醇湿粘接、水湿粘接和水过湿粘接方案进行粘接。将复合-牙本质板在含有双仿生模拟物的介质中进行再矿化 1-4 个月，以波特兰水泥为钙源，并通过透射电子显微镜进行表征。在乙醇湿粘接的标本中，再矿化要么不存在，要么仅限于纤维内模式。在水湿粘接的标本中观察到广泛的纤维内和纤维间再矿化。水过湿标本表现出混合层的部分再矿化和水通道内矿化板的沉淀。使用乙醇湿粘接证实了仿生再矿化是一个渐进的脱水过程，用磷灰石晶体取代混合层中的残留水。
