Marongiu Fabio, Gramignoli Roberto, Sun Qian, Tahan Veysel, Miki Toshio, Dorko Kenneth, Ellis Ewa, Strom Stephen C
University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
Curr Protoc Stem Cell Biol. 2010 Mar;Chapter 1:Unit 1E.5. doi: 10.1002/9780470151808.sc01e05s12.
Mesenchymal stem cells (MSCs) have the ability to differentiate into osteocytes, chondrocytes, and adipocytes and possess immunomodulatory properties. Amniotic membrane from human term placenta is a potential source of multipotent MSCs that could be useful for regenerative medicine. This unit describes a detailed and simple protocol for the isolation of amniotic mesenchymal cells. We also introduce a simple density separation technique for the purification of this cell type from possible contamination with amniotic epithelial cells.
间充质干细胞（MSCs）具有分化为骨细胞、软骨细胞和脂肪细胞的能力，并具有免疫调节特性。人足月胎盘的羊膜是多能间充质干细胞的潜在来源，可用于再生医学。本单元描述了一种分离羊膜间充质细胞的详细且简单的方案。我们还介绍了一种简单的密度分离技术，用于从可能被羊膜上皮细胞污染的情况中纯化这种细胞类型。
