3rd University Obstetrics and Gynaecology Clinic, Ippokration General Hospital, Medical School, Aristotle University Thessaloniki, Thessaloniki, Greece.
Transfusion. 2011 May;51(5):976-85. doi: 10.1111/j.1537-2995.2011.03077.x. Epub 2011 Mar 7.
Placenta is a valuable source of stem cells for cell therapy and future application in the field of regenerative medicine. This is due to the plasticity and the immunomodulatory effects of the stem cells that it contains. In this study we present a totally closed method for hematopoietic and nonhematopoietic stem cell isolation from human term placenta.
Sixty-eight placenta units were collected and manipulated for the residual fetal blood drainage. After delivery, placenta flushing with citrate-phosphate-dextrose-adenine was evaluated.
Placenta flushing using a totally closed system led to a significant amount of hematopoietic progenitor cells and multipotent mesenchymal stem cells (MSCs) without additional microbial risk, free of maternal cell contamination.
Traditionally discarded after childbirth, the term placenta now appears to be an easily accessible and abundant source of diverse origin stem cells suitable for banking strategies and for future clinical applications, including adult therapy.
胎盘是细胞治疗和再生医学领域未来应用的有价值的干细胞来源。这是由于它所包含的干细胞的可塑性和免疫调节作用。在这项研究中，我们提出了一种完全封闭的方法，用于从人足月胎盘分离造血和非造血干细胞。
收集了 68 个胎盘单位，并对其进行了残余胎儿血液引流操作。分娩后，评估了柠檬酸盐-磷酸盐-葡萄糖-腺嘌呤对胎盘的冲洗效果。
使用完全封闭系统对胎盘进行冲洗，可获得大量造血祖细胞和多能间充质干细胞（MSCs），而不会增加微生物风险，也不会有母体细胞污染。
胎盘在分娩后通常被丢弃，但现在看来，它是一种易于获得且丰富的多能干细胞来源，适合储存策略和未来的临床应用，包括成人治疗。
