Aprotosoaie Ana Clara, Hăncianu Monica, Poiată Antonia, Tuchiluş Cristina, Spac A, Cioană Oana, Gille Elvira, Stănescu Ursula
School of Pharmacy, Department of Pharmacognosy, "Gr.T. Popa" University of Medicine and Pharmacy Iaşi.
Rev Med Chir Soc Med Nat Iasi. 2008 Jul-Sep;112(3):832-6.
In our study, four samples of volatile oil from Foeniculum vulgare, cultivated in different pedoclimatic conditions, were investigated for their antimicrobial activity and chemical composition.
Organisms. Staphylococcus aureus ATCC 25923, Bacillus cereus, Pseudomonas aeruginosa, Escherichia coli ATCC 25922, Candida albicans were included in the report. Antimicrobial susceptibility tests. The comparative inhibitory activity of volatile oil samples with other antimicrobial agents was quantitative determined by minimum inhibitory concentration (MIC). Oil samples are the volatile oils extracted by steam distillation, from two ecological vegetative populations of Foeniculum vulgare. Gas chromatography coupled to mass spectrometry (GC-MS) was used to determine the chemical composition of the essential oils.
All oil samples have a good activity against E. coli and S. aureus at low concentrations. Against B. cereus and P. aeruginosa these oil samples are less active. The oil samples were generally bactericidal at a concentration up to twofold or fourfold higher than the MIC value. Significantly synergic activity with amoxicillin or tetracycline showed all fennel samples against E. coli, Sarcina lutea and B. subtilis strains. Fennel oil samples have shown high activity against Candida albicans.
No significant antimicrobial activity variations were observed for Foeniculum vulgare volatile oil samples obtained after two or three years cultivation period. The most important identified compounds in all samples of fennel volatile oils were trans-anethole, estragole, fenchone, limonene, alpha-pinene and gamma-terpinene.
在我们的研究中,对在不同土壤气候条件下种植的4份小茴香挥发油样品进行了抗菌活性和化学成分研究。
微生物。报告中纳入了金黄色葡萄球菌ATCC 25923、蜡样芽孢杆菌、铜绿假单胞菌、大肠埃希菌ATCC 25922、白色念珠菌。抗菌药敏试验。通过最低抑菌浓度(MIC)定量测定挥发油样品与其他抗菌剂的比较抑菌活性。油样是通过水蒸气蒸馏从两个生态植物种群的小茴香中提取的挥发油。采用气相色谱-质谱联用(GC-MS)测定精油的化学成分。
所有油样在低浓度下对大肠埃希菌和金黄色葡萄球菌均有良好活性。对蜡样芽孢杆菌和铜绿假单胞菌,这些油样活性较低。油样在浓度高达MIC值的两倍或四倍时通常具有杀菌作用。所有小茴香样品对大肠埃希菌、藤黄八叠球菌和枯草芽孢杆菌菌株与阿莫西林或四环素均表现出显著的协同活性。小茴香油样对白色念珠菌表现出高活性。
两年或三年种植期后获得的小茴香挥发油样品未观察到显著的抗菌活性差异。所有小茴香挥发油样品中鉴定出的最重要化合物为反式茴香脑、草蒿脑、小茴香酮、柠檬烯、α-蒎烯和γ-松油烯。