Biomedical Engineering Center, College of Chemistry and Chemical Engineering, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, PR China.
J Biomed Nanotechnol. 2009 Oct;5(5):565-72. doi: 10.1166/jbn.2009.1062.
We performed single molecule fluorescence detection of YOYO-1 labeled lambda-DNA molecules adsorbed on a solid substrate after treatment with different concentrations of agarose by total internal reflection fluorescence microscopy (TIRFM). At agarose-modified surfaces, we found that molecular adsorption behaviors were dependent on agarose concentration, pH and ionic strength. We further revealed the subtle differences among adsorptive forces. Both electrostatic interaction and hydrophobic interaction were the major driving force for adsorption of DNA molecules on agarose surfaces. We also found that surface topography plays a role to a certain extent in the adsorption dynamics. The characteristics of agarose surfaces were further assessed using contact angle (CA) measurements.
我们通过全内反射荧光显微镜（TIRFM）对不同浓度琼脂糖处理后吸附在固体基底上的 YOYO-1 标记的 λ-DNA 分子进行单分子荧光检测。在琼脂糖修饰的表面上，我们发现分子吸附行为取决于琼脂糖浓度、pH 值和离子强度。我们进一步揭示了吸附力之间的细微差别。静电相互作用和疏水相互作用都是 DNA 分子在琼脂糖表面吸附的主要驱动力。我们还发现表面形貌在一定程度上对吸附动力学起着作用。使用接触角（CA）测量进一步评估了琼脂糖表面的特性。
