USDA-ARS, USA.
J Agric Food Chem. 2010 Apr 14;58(7):3977-83. doi: 10.1021/jf9030772.
Anthocyanins (ACNs) from various foods have been shown to minimize the development of obesity in some animal models. The objective of the current study was to compare the effects of feeding purified black raspberry (BRB) ACNs or the freeze-dried whole BRB on the development of obesity. Male C57BL/6J mice (25 days of age) were assigned at random to treatments (7/treatment; 3/cage). The treatments included (1) control low-fat diet (10% calories from fat) (LF); (2) LF plus BRB juice in place of drinking water; (3) LF diet plus purified BRB ACNs in drinking water (1.25 mg/mL); (4) control high-fat diet (60% calories from fat) (HF60); (5) HF60 diet + BRB juice in place of drinking water; (6) HF60 diet + ACNs in drinking water (1.25 mg/mL); and (7) HF60 + freeze-dried whole BRB powder (21.7 g/kg of diet). Body weight gains in mice fed HF60 diet plus purified BRB ACNs tended to be lower after 56, 63, and 70 days than in mice fed HF60 alone. Body weights were increased at time of sacrifice, but heart, liver, and kidney weights as a percentage of body weight were decreased in mice fed HF60 diet compared to LF fed mice. Weights (g or g/body weight) of epididymal and retroperitoneal fat were increased in the HF60 fed mice compared to LF fed mice. Fasting serum glucose, leptin, and insulin levels as well as homeostasis assessment of insulin resistance (HOMA-IR) were elevated in mice fed the HF60 diet relative to LF-fed controls. Serum cholesterol, triglycerides, and monocyte chemoattractant protein-1 (MCP-1) were not altered by diet. Serum levels of resistin were increased in mice fed the HF60 diet compared to mice fed the LF diet. None of the responses measured were altered by whole BRB powder included in the diet relative to the HF60 control diet. Cyanidin containing di- or triglycosides in BRB was ineffective in altering the development of obesity in contrast to cyanidin-monoglycosides, which have been shown to be effective. The sugar moiety on the anthocyanidins may be an important factor in determining the response in the development of obesity.
各种食物中的花色苷（ACNs）已被证明可在某些动物模型中最大程度地减少肥胖的发展。本研究的目的是比较喂养纯化的黑莓（BRB）ACNs 或冻干全 BRB 对肥胖发展的影响。25 天大的雄性 C57BL/6J 小鼠（7 只/处理；3 只/笼）被随机分配到处理组（3 组/笼）。处理组包括（1）对照低脂饮食（10%卡路里来自脂肪）（LF）；（2）LF 加黑莓汁代替饮用水；（3）LF 饮食加纯化 BRB ACNs 在饮用水中（1.25 mg/mL）；（4）对照高脂肪饮食（60%卡路里来自脂肪）（HF60）；（5）HF60 饮食+黑莓汁代替饮用水；（6）HF60 饮食+饮用水中的 ACNs（1.25 mg/mL）；和（7）HF60+冻干全 BRB 粉（21.7 g/kg 饮食）。与单独喂食 HF60 的小鼠相比，喂食 HF60 饮食加纯化 BRB ACNs 的小鼠在 56、63 和 70 天后的体重增加趋势较低。在处死时，体重增加，但与 LF 喂养的小鼠相比，HF60 饮食喂养的小鼠的心脏、肝脏和肾脏重量占体重的百分比降低。与 LF 喂养的小鼠相比，HF60 喂养的小鼠附睾和腹膜后脂肪的重量（g 或 g/体重）增加。与 LF 喂养的对照相比，喂食 HF60 饮食的小鼠的空腹血清葡萄糖、瘦素和胰岛素水平以及胰岛素抵抗的稳态评估（HOMA-IR）升高。血清胆固醇、甘油三酯和单核细胞趋化蛋白-1（MCP-1）不受饮食影响。与 LF 饮食相比，喂食 HF60 饮食的小鼠的抵抗素水平升高。与 HF60 对照饮食相比，饮食中包含的全 BRB 粉对测量的任何反应均无影响。与已证明有效的花青素单糖苷相比，BRB 中含二糖苷或三糖苷的氰定没有改变肥胖的发展。花色苷上的糖部分可能是决定肥胖发展反应的一个重要因素。
