Agriculture Research Service, U.S. Department of Agriculture, Arkansas Children's Nutrition Center, Little Rock, Arkansas 72202, USA.
J Agric Food Chem. 2010 Apr 14;58(7):3970-6. doi: 10.1021/jf902852d.
Male C57BL/6J mice (25 days of age) were fed either a low-fat diet (10% kcal from fat) (LF) or a high-fat diet (45% kcal from fat) (HF45) for a period of 72 days. Blueberry juice or purified blueberry anthocyanins (0.2 or 1.0 mg/mL) in the drinking water were included in LF or HF45 treatments. Sucrose was added to the drinking water of one treatment to test if the sugars in blueberry juice would affect development of obesity. Total body weights (g) and body fat (%) were higher and body lean tissue (%) was lower in the HF45 fed mice compared to the LF fed mice after 72 days, but in mice fed HF45 diet plus blueberry juice or blueberry anthocyanins (0.2 mg/mL), body fat (%) was not different from those mice fed the LF diet. Anthocyanins (ACNs) decreased retroperitoneal and epididymal adipose tissue weights. Fasting serum glucose concentrations were higher in mice fed the HF45 diet. However, it was reduced to LF levels in mice fed the HF45 diet plus 0.2 mg of ACNs/mL in the drinking water, but not with blueberry juice. beta cell function (HOMA-BCF) score was lowered with HF45 feeding but returned to normal levels in mice fed the HF45 diet plus purified ACNs (0.2 mg/mL). Serum leptin was elevated in mice fed HF45 diet, and feeding either blueberry juice or purified ACNs (0.2 mg/mL) decreased serum leptin levels relative to HF45 control. Sucrose in drinking water, when consumption was restricted to the volume of juice consumed, produced lower serum leptin and insulin levels, leptin/fat, and retroperitoneal and total fat (% BW). Blueberry juice was not as effective as the low dose of anthocyanins in the drinking water in preventing obesity. Additional studies are needed to determine factors responsible for the differing responses of blueberry juice and whole blueberry in preventing the development of obesity.
雄性 C57BL/6J 小鼠（25 日龄）分别喂食低脂饮食（10%热量来自脂肪）（LF）或高脂肪饮食（45%热量来自脂肪）（HF45）72 天。蓝莓汁或纯化的蓝莓花青素（0.2 或 1.0mg/mL）添加到 LF 或 HF45 处理的饮用水中。在一种处理中，在饮用水中添加蔗糖以测试蓝莓汁中的糖是否会影响肥胖的发展。与 LF 喂养的小鼠相比，72 天后，HF45 喂养的小鼠的总体重（g）和体脂（%）更高，体瘦肉组织（%）更低，但 HF45 饮食加蓝莓汁或蓝莓花青素（0.2mg/mL）喂养的小鼠的体脂（%）与 LF 饮食喂养的小鼠无差异。花青素（ACNs）降低了腹膜后和附睾脂肪组织的重量。HF45 饮食喂养的小鼠空腹血清葡萄糖浓度升高。然而，在饮用水中添加 0.2mg/mL 的 ACNs 时，HF45 饮食喂养的小鼠的血糖浓度降低至 LF 水平，但添加蓝莓汁时则没有。HF45 喂养降低了β细胞功能（HOMA-BCF）评分，但 HF45 饮食加纯化 ACNs（0.2mg/mL）喂养的小鼠的评分恢复正常。HF45 饮食喂养的小鼠血清瘦素升高，而添加蓝莓汁或纯化 ACNs（0.2mg/mL）则降低了血清瘦素水平，与 HF45 对照组相比。饮用水中的蔗糖，当消耗限制在果汁的体积时，会降低血清瘦素和胰岛素水平、瘦素/脂肪比、腹膜后和总脂肪（BW%）。蓝莓汁在预防肥胖方面不如饮用水中的低剂量花青素有效。需要进一步的研究来确定蓝莓汁和整个蓝莓在预防肥胖发展方面反应不同的原因。
