猪传染性胃肠炎病毒中国野毒株基因3区序列分析

Analysis of the gene 3 region sequences of Chinese field strains of Transmissible gastroenteritis virus.

作者信息

Wang C B, Feng L, Chen J F, Shi H Y, Qiu H J, Xue F, Zhu Y M, Liu S W

机构信息

Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, P.R. China.

出版信息

Acta Virol. 2010;54(1):61-73. doi: 10.4149/av_2010_01_61.

DOI:10.4149/av_2010_01_61

PMID:20201616

Abstract

The genome of Transmissible gastroenteritis virus (TGEV) displays genetic diversity especially in gene 3 region. Sequence and comparative analysis of 3a and 3b genes of eight Chinese field strains with reference TGEV strains indicated that these strains shared 87.0-100% and 51.5%-100% identities at the nucleotide level, respectively, and 86.1%-100% and 66.2%-100% identities at the amino acid level, respectively. Moreover, in one of the strains (CH/SDQ/08), a 51 nt deletion in the gene 3 region was found. Phylogenetic analysis showed that the eight Chinese strains were more closely related to TGEV strains H165, H16, Miller M6, Miller M60, TS, and CHV than to other reference strains. In addition, this study indicated the presence of different TGEV strains within the same pig herds in China.

摘要

传染性胃肠炎病毒（TGEV）的基因组表现出遗传多样性，尤其是在基因3区域。对8株中国田间毒株与参考TGEV毒株的3a和3b基因进行序列和比较分析表明，这些毒株在核苷酸水平上的同一性分别为87.0%-100%和51.5%-100%，在氨基酸水平上的同一性分别为86.1%-100%和66.2%-100%。此外，在其中一株毒株（CH/SDQ/08）中，发现基因3区域有一个51 nt的缺失。系统发育分析表明，这8株中国毒株与TGEV毒株H165、H16、Miller M6、Miller M60、TS和CHV的亲缘关系比与其他参考毒株更近。此外，本研究表明在中国同一猪群中存在不同的TGEV毒株。