Laboratory of Immunology, Posgraduate Division, Faculty of Medicine Dr. Ignacio Chávez, University of San Nicolás De Hidalgo, Morelia, Michoacán, México.
Mini Rev Med Chem. 2010 Dec;10(14):1393-400. doi: 10.2174/138955710793564142.
Chemiluminescence has traditionally been used to study the nature of the oxidative bactericidal mechanisms of neutrophils and monocytes, the intrinsic defects of abnormally functioning neutrophils or monocytes and cell activation. During the last ten years Chemiluminescence has been applied in a wide variety of techniques including: immunoassays, protein blotting, toxicological and pharmacological tests (e.g. after exposure to antibiotic or immunomodulators agents such as adjuvants and cytotoxic drugs). In this review, we discuss some promising clinical applications of Chemiluminescence in clinical immunology for the study of autoimmune diseases, inflammatory responses, endocrine disorders, immunodeficiency's states, mucosal immune response against drugs and pathogens and host responses against tumors and infections. Further, we review numerous advantages showed by Chemiluminescence-based methods over other methods to assay the same endpoints, which facilitate their uses in the current practice of clinical immunology.
化学发光技术传统上被用于研究中性粒细胞和单核细胞的氧化杀菌机制的本质、功能异常的中性粒细胞或单核细胞的固有缺陷以及细胞激活。在过去的十年中，化学发光已被应用于多种技术，包括：免疫测定、蛋白印迹、毒理学和药理学试验（例如，在接触抗生素或免疫调节剂如佐剂和细胞毒性药物后）。在这篇综述中，我们讨论了化学发光在临床免疫学中的一些有前途的临床应用，用于研究自身免疫性疾病、炎症反应、内分泌紊乱、免疫缺陷状态、针对药物和病原体的黏膜免疫反应以及宿主对肿瘤和感染的反应。此外，我们还回顾了基于化学发光的方法在检测相同终点方面优于其他方法的许多优点，这使得它们在临床免疫学的当前实践中得到了广泛应用。
