Li Na, Liu Danqing, Cui Hua
State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation, Chengdu University of Technology, Chengdu, 610059, Sichuan, China.
Anal Bioanal Chem. 2014 Sep;406(23):5561-71. doi: 10.1007/s00216-014-7901-x. Epub 2014 Jun 8.
Chemiluminescence-based bioassays have become increasingly important in clinical, pharmaceutical, environmental, and food safety fields owing to their high sensitivity, wide linear range, and simple instrumentation. During the past decade, it has been found that metal nanoparticles can initiate various liquid-phase chemiluminescence reactions as catalysts, reductants, energy acceptors, and nanosized reaction platforms owing to their unique optical, catalytic, and surface properties and chemical reactivity, which are very important for chemiluminescence bioassays based on metal nanoparticles as nanoprobes or a nanointerface. In this article, we summarize recent progress in metal-nanoparticle-initiated liquid-phase chemiluminescence, including reaction systems, mechanisms, and their applications in chemiluminescence-based bioassays, especially for immunoassays, DNA assays, aptamer-based assays, high-performance liquid chromatography or capillary electrophoresis analysis, and flow injection analysis.
基于化学发光的生物分析方法因其高灵敏度、宽线性范围和简单的仪器设备，在临床、制药、环境和食品安全领域变得越来越重要。在过去十年中，人们发现金属纳米颗粒由于其独特的光学、催化和表面性质以及化学反应性，能够作为催化剂、还原剂、能量受体和纳米级反应平台引发各种液相化学发光反应，这对于基于金属纳米颗粒作为纳米探针或纳米界面的化学发光生物分析非常重要。在本文中，我们总结了金属纳米颗粒引发的液相化学发光的最新进展，包括反应体系、机理及其在基于化学发光的生物分析中的应用，特别是在免疫分析、DNA分析、适体分析、高效液相色谱或毛细管电泳分析以及流动注射分析中的应用。
