Arany Szilvia, Ohtani Susumu
Department of Biochemistry, Akita University School of Medicine, Akita, Japan.
J Forensic Sci. 2010 May;55(3):701-5. doi: 10.1111/j.1556-4029.2010.01324.x. Epub 2010 Feb 25.
Here, we report on an experimental approach of simultaneous determination of various amino acids racemization (AAR) rates in teeth. We evaluated the measurements of aspartic acid (Asp), glutamate (Glu), and alanine (Ala) isolated from dentin. Asx D/L rates from total amino acid fraction, generally used for age estimation, showed high correlation (r = 0.98) with age. As Glx and Ala showed very slow racemization kinetics in TA, we performed further analysis of the acid-soluble protein (SP) fraction. The results supported improved correlation between age and D/L rates for Glu (r = 0.84) and Ala (r = 0.85), as well as for Asp (r = 0.98). By providing further elucidation on dentin protein racemization, the technique offers a considerable opportunity to involve other amino acids in age estimation studies. As the process does not require additional separation steps, the method can be easily adapted to existing protocols.
在此，我们报告一种同时测定牙齿中各种氨基酸消旋化（AAR）速率的实验方法。我们评估了从牙本质中分离出的天冬氨酸（Asp）、谷氨酸（Glu）和丙氨酸（Ala）的测量值。通常用于年龄估计的总氨基酸组分中的Asx D/L速率与年龄显示出高度相关性（r = 0.98）。由于Glx和Ala在牙本质中显示出非常缓慢的消旋化动力学，我们对酸溶性蛋白（SP）组分进行了进一步分析。结果支持了年龄与Glu（r = 0.84）、Ala（r = 0.85）以及Asp（r = 0.98）的D/L速率之间更好的相关性。通过对牙本质蛋白消旋化提供进一步阐释，该技术为在年龄估计研究中纳入其他氨基酸提供了相当大的机会。由于该过程不需要额外的分离步骤，该方法可以很容易地适用于现有方案。
